Enzyme-catalyzed reactions, or biocatalysis, have emerged as a transformative tool in pharmaceutical R&D, offering unparalleled chemo-, regio-, and stereoselectivity under mild reaction conditions. Unlike traditional chemical synthesis, enzymatic pathways significantly reduce the reliance on toxic organic solvents and heavy metals, providing a greener, more sustainable route to complex molecules. BOC Sciences leverages an extensive enzyme library and advanced protein engineering technologies to deliver comprehensive biocatalysis services. We assist clients in constructing difficult chiral centers, achieving selective functionalization, and optimizing synthetic routes from milligram-scale screening to kilogram-scale process demonstration, accelerating the discovery and development of novel therapeutics.
We leverage an extensive proprietary library of thousands of off-the-shelf enzymes (KREDs, ATAs, IREDs, Lipases, etc.) to rapidly identify "hits" for your specific substrate. Our automated HTS platform ensures fast turnaround times, evaluating activity and enantioselectivity to select the best starting biocatalyst for your reaction.
When natural enzymes lack the required performance, we employ rational design and directed evolution strategies. We optimize enzyme properties—such as substrate specificity, thermostability, solvent tolerance, and stereocontrol—to create a robust biocatalyst tailored to your specific process conditions.
Bridging the gap between biology and chemistry, we optimize critical reaction parameters (pH, temperature, cofactor regeneration, substrate loading). Our experts design scalable biphasic or monophasic systems to maximize conversion rates and space-time yields while minimizing waste.
To enhance enzyme stability and enable recyclability, we offer screening of various immobilization carriers and methods (adsorption, covalent binding, entrapment). This service is essential for reducing catalyst costs and facilitating continuous flow chemistry applications.
We provide seamless scale-up of enzyme production from shake flasks to high-density fermentation. Our downstream processing capabilities ensure the delivery of high-activity enzyme preparations (lyophilized powders or liquid formulations) suitable for kilogram-scale synthesis campaigns.
We integrate enzymatic steps into traditional chemical routes to solve complex synthetic challenges. Whether it is constructing chiral centers or achieving regioselective functionalization, we deliver high-purity intermediates and APIs by combining the best of biological and chemical catalysis.
BOC Sciences harnesses the power of enzymes to solve complex synthetic challenges with high stereocontrol and atom economy.
Common Enzymes: Lipases (e.g., CAL-B), Esterases, Proteases, Amidases, Epoxide Hydrolases.
Applications: Widely used for kinetic resolution, desymmetrization of precursors, and resolution of racemic mixtures; the most mature class in industrial biocatalysis.
Common Enzymes: Ketoreductases (KREDs), Alcohol Dehydrogenases, Monooxygenases (e.g., P450), Oxidases.
Applications: Essential for preparing chiral alcohols, constructing difficult chiral centers, and achieving highly selective regio- or stereoselective hydroxylation.
Common Enzymes: Transaminases (ATAs), Glycosyltransferases, Kinases.
Applications: The primary tool for the biocatalytic synthesis of chiral amines, as well as carbohydrate modification and library construction.
Common Enzymes: Aldolases, Nitrile Hydratases, Decarboxylases.
Applications: Used for Carbon-Carbon (C-C) bond formation, green synthesis of amides from nitriles, and decarboxylation reactions with high atom economy.
Common Enzymes: Racemases, Epimerases, Cis-trans Isomerases.
Applications: Crucial for inverting chiral center configurations (e.g., facilitating racemization in Dynamic Kinetic Resolution) and adjusting molecular stereochemistry.
Common Enzymes: DNA Ligases, Aminoacyl-tRNA Synthetases.
Applications: Primarily utilized in molecular biology and genetic engineering workflows; applied in specific high-value biosynthetic pathways.
BOC Sciences provides a versatile biocatalysis platform covering a wide range of chemical transformations to address diverse synthetic challenges.
Submit your reaction scheme or target molecule structure. Our biocatalysis experts will evaluate feasibility and propose a tailored screening or optimization plan.
We analyze your target molecule and propose potential enzymatic disconnections to replace difficult chemical steps, defining the project scope and feasibility.
Using High-Throughput Enzyme Screening, we test panels of enzymes against your substrate to identify "hits" with initial activity and selectivity.
We optimize reaction conditions (solvent, pH, temp) and perform enzyme evolution if necessary to improve conversion rates and enantiomeric excess.
The optimized process is scaled up to produce the required amount of compound (grams to kgs), followed by rigorous analytical characterization before delivery.
We accelerate hit-to-lead optimization by providing rapid access to chiral building blocks and difficult-to-synthesize metabolites. Our high-throughput screening identifies biocatalysts that unlock novel chemical space, enabling the fast synthesis of enantiopure compounds at the milligram scale for early structure-activity relationship (SAR) studies without the need for lengthy method development.
We transform synthetic routes into scalable, cost-effective bioprocesses. Our team optimizes reaction parameters and engineers enzymes for stability under harsh industrial conditions, delivering robust routes that reduce step counts, minimize hazardous waste, and ensure safety during kilogram-to-ton scale manufacturing, seamlessly bridging the gap between bench and pilot plant.
We design competitive chemo-enzymatic routes to bypass patent roadblocks and reduce cost of goods (COGS). By replacing expensive transition metal catalysts and complex protection/deprotection steps with highly selective enzymes, we help clients achieve higher yields and purities while strictly aligning with green chemistry and sustainability goals.
For innovative companies with limited internal infrastructure, we act as your dedicated external biocatalysis department. From initial retrosynthetic analysis to final product delivery, we provide end-to-end support and expert consultation, allowing you to leverage advanced enzyme technologies to fast-track your lead candidates without heavy capital investment in equipment or personnel.
Partner with BOC Sciences to access state-of-the-art enzyme technologies. Whether you need a single screening experiment or a fully developed chemo-enzymatic route, our team ensures precision, speed, and confidentiality.
Access to a vast proprietary collection of enzymes covering all major catalytic classes, ensuring a high probability of finding the right "hit" for your specific transformation.
We don't just screen; we improve. Our capabilities in bioinformatics and directed evolution allow us to tailor enzymes to non-natural substrates and harsh process conditions.
Our facility is equipped for both micro-scale screening and preparative scale synthesis, ensuring a smooth transition from proof-of-concept to material delivery for your studies.
Client Needs: A client needed a scalable route to a chiral amine intermediate with strict optical purity requirements (>99.5% ee). The existing chemical reductive amination route used an expensive chiral metal catalyst and resulted in heavy metal residue issues.
Challenges: The substrate had low solubility in aqueous buffers, and the equilibrium of the reaction favored the ketone starting material. Furthermore, the desired amine had to be produced with high diastereoselectivity.
Solution: BOC Sciences deployed a rapid high-throughput screening campaign across our proprietary Transaminase (ATA) library to identify a high-activity variant. To overcome thermodynamic limitations, our process chemists engineered a robust biphasic reaction system coupled with an isopropylamine amine donor strategy, successfully shifting the equilibrium toward the product while ensuring full substrate solubility.
Outcome: We successfully delivered 500g of the chiral amine with >99.8% ee and >90% isolated yield. The process eliminated metal contaminants and reduced the overall cost of goods by 40%.
Client Needs: A medicinal chemistry team required the synthesis of a specific hydroxylated metabolite of a lead compound to assess its toxicology profile. Chemical synthesis was not feasible due to lack of regioselectivity.
Challenges: The molecule contained multiple oxidizable sites (aromatic rings and aliphatic chains). The goal was to selectively hydroxylate a specific unactivated methylene carbon without over-oxidation.
Solution: Leveraging our diverse P450 monooxygenase platform, we identified a candidate capable of distinguishing between multiple similar C-H bonds. Through rational active-site engineering, we further enhanced its regioselectivity, establishing a direct, single-step enzymatic hydroxylation route that bypassed the complex protection-deprotection sequences required by traditional organic synthesis.
Outcome: Provided 100mg of the pure metabolite within 4 weeks. The enzymatic reaction proceeded in a single step, bypassing a proposed 8-step chemical synthesis route.
Client Needs: Synthesis of a chiral ester from a racemic alcohol. Standard kinetic resolution would limit the theoretical yield to 50%, which was economically unacceptable for this high-value intermediate.
Challenges: Developing a DKR system where the unreactive enantiomer is continuously racemized while the reactive enantiomer is acylated by the enzyme.
Solution: We designed a sophisticated chemo-enzymatic DKR system by coupling an immobilized Lipase (CAL-B) with a compatible ruthenium-based racemization catalyst. Our team meticulously optimized the reaction kinetics to synchronize the rate of chemical racemization with enzymatic acylation, effectively overcoming the 50% yield ceiling inherent in classical kinetic resolutions.
Outcome: Achieved a conversion of >95% and an ee of >99% for the desired chiral ester, effectively breaking the 50% yield barrier of traditional resolution and maximizing atom economy.
Substrate selection directly affects enzyme catalytic efficiency and specificity. Systematic screening of different structures and functional groups, combined with enzyme kinetics analysis, can identify the most suitable system. BOC Sciences provides professional substrate screening and custom services, supporting high-throughput evaluation and structural optimization to accelerate research and industrial applications.
Temperature significantly influences enzyme activity and stability; extreme conditions can reduce catalytic efficiency. By optimizing temperature curves and analyzing thermal stability, the best reaction conditions can be determined. BOC Sciences assists clients with temperature-dependence evaluation and reaction optimization to ensure stable and reproducible enzyme-catalyzed processes.
Substrate concentration affects reaction rate and enzyme kinetics; too high may cause inhibition, too low reduces conversion. Through Michaelis-Menten kinetics and reaction modeling, optimal concentrations can be accurately determined. BOC Sciences offers customized experimental design and kinetic evaluation services to achieve efficient, stable enzyme-catalyzed conversions at lab or industrial scale.
Solvent choice impacts enzyme solubility, substrate accessibility, and reaction rate. Typically, enzyme-compatible, low-inhibition solvents are preferred, and solvent screening experiments optimize conditions. BOC Sciences provides solvent effect assessment and system optimization services to maintain enzyme activity and high conversion under various conditions.
Conversion depends on enzyme amount, substrate concentration, reaction time, and cofactors. Systematic optimization of these parameters can significantly increase efficiency. Additionally, enzyme engineering or immobilization enhances stability and reusability. BOC Sciences offers comprehensive support from kinetic optimization to process scale-up, ensuring high-efficiency, controllable enzyme-catalyzed processes for clients.
The enzyme screening service provided by BOC Sciences was a game-changer for our project. They identified a hydrolase that resolved our racemic mixture with perfect enantioselectivity, saving us months of chiral chromatography development.
— Dr. William, Senior Scientist, Biotechnology Company
We wanted to move away from metal catalysts for our scale-up. BOC Sciences successfully developed a transaminase route that was not only greener but also cleaner, simplifying our downstream purification significantly.
— Dr. Daniel, Director of Process Chemistry, Pharmaceutical Company
Their ability to engineer enzymes for non-natural substrates is impressive. The team at BOC Sciences delivered a customized ketoreductase that worked on our sterically hindered substrate where commercial enzymes failed.
— Dr. Emily, VP of R&D, Biopharmaceutical Company
From the initial consultation to the final delivery of the compound, the communication was excellent. They provided detailed reports on the screening and optimization data, which helped us understand the process boundaries.
— Dr. James, Senior Research Chemist, Research Institute
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