Flash Column Chromatography

Flash Column Chromatography

Flash column chromatography is a faster method of separating components of a mixture, which has become an extremely useful and popular means of purification of small as well as large quantities of compounds. The technique is sufficiently simple and powerful that a beginner can successfully resolve diastereomers on sample scales ranging from 100 mg to >1 g. Since its description in 1978, flash column chromatography has become a routinely used tool to separate fine chemicals in the organic laboratory. For example, pure R-amino acid-N-carboxyanhydride (NCA) monomers which are the widely used precursors for the synthesis of polypeptides, can be obtained using the flash column chromatography, without the need for recrystallization.

Advantages of Flash Column Chromatography

Traditional column chromatography is an important purification separation method used in many laboratories. Unlike flash column chromatography, it can be very time consuming and labor intensive though. Flash column chromatography systems speed up the process by using pressurized gases to rapidly move eluents through the column. These column separation solutions can pump as many as four types of solvents at rates up to 250ml/min, at up to 10bar/145psi. A flash chromatography system typically uses a UV or IR detector, perfect for precisely analyzing separation peaks. Besides, the flash column chromatography systems can be reconfigured due to their modular design.

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Macroporous Adsorption Resin

Macroporous adsorption resin uses a special adsorbent to selectively adsorb the active ingredient from compound decoction of crude drugs, and remove the invalid component. The method is simple in equipment, convenient in operation, energy-saving, low in cost, high in product purity, and non-hygroscopic. Therefore, the technology has been widely used for the purification of glycosides, flavonoids, and alkaloids.

The adsorption of ZMS is a physical process. The main reason for the adsorption is a kind of “surface force” produced by the molecular gravitational action on the solid surface. When the fluid flows through, some molecules in the fluid collide with the adsorbent surface due to irregular movement, resulting in molecular enrichment on the surface, so that the number of molecules in the fluid is reduced and separation and elimination are achieved. As the adsorption does not undergo chemical changes, as long as the molecules on the surface gradually disappear, the ZMS has the ability to absorb compounds again. It can be reused to reduce the costs, so it is widely used in the petrochemical industry, detergent industry, fine chemicals and other industries.

Why Choose BOC Sciences?

BOC Sciences has advanced technology and good quality. Our experienced researchers who are familiar with the properties of various substances, will do the purification according to your requirements and 100% guaranteed service for customers. On the one hand, we have the appropriate equipment and technology to separate and purify the products for you. On the other hand, we can connect the flash column chromatography with UV spectrum or IR detector to provide better analysis for the mixed products.


  1. Fair, J. D., & Kormos, C. M. (2008). Flash column chromatograms estimated from thin-layer chromatography data. Journal of Chromatography A, 1211(1-2), 49-54.
  2. Pedersen, D. S., & Rosenbohm, C. (2001). Dry column vacuum chromatography. Synthesis, 2001(16), 2431-2434.
  3. Edwards, C., Lawton, L. A., Coyle, S. M., & Ross, P. (1996). Laboratory-scale purification of microcystins using flash chromatography and reversed-phase high-performance liquid chromatography. Journal of Chromatography A, 734(1), 163-173.
  4. Kramer, J. R., & Deming, T. J. (2010). General method for purification of α-amino acid-N-carboxyanhydrides using flash chromatography. Biomacromolecules, 11(12), 3668-3672.

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