High-content Screening
Drug discovery is a labor-intensive industry, and drug screening is the only way for the early stage of the drug discovery. It refers to the method to detect the possible pharmacological activity of chemical compounds, protein, peptides and natural products by applying appropriate screening methods and technologies, so as to provide experimental basis for the development of new drugs. Drug screening is an important link connecting drugs from laboratory research to clinical application, and it is also the key to make new drug research continue.
With the development of high-throughput technology, the single target screening method has been unable to meet the needs of drug discovery, and is not conducive to the comprehensive evaluation of biological activities of compounds. Therefore, high-content screening technology with multi-indicators and multi-targets interaction as the main feature was developed. HCS model is mainly established at the cell level. By observing the effects of samples on fixed or dynamic cell morphology, growth, differentiation, migration, apoptosis, metabolism, signal transduction and other functions, involving the target of cell membrane receptors, cell components, organelles, etc., the effects of samples are analyzed from multiple perspectives, and the activity and possible toxicity of samples are finally determined. BOC Sciences can provides the high-content screening services to support your drug discovery process.
High-content screening (HCS, also known as high-content analysis, or HCA) is an approach to cell biology that combines automated imaging and quantitative data analysis in a high-throughput format suitable for large-scale applications such as drug discovery and systems biology. The process of HCS typically involves treating cells or biological samples with a variety of compounds, dyes, or other markers, and then imaging them using automated microscopes. The resulting images are then analyzed using software to extract quantitative data on a variety of cellular parameters, such as cell morphology, protein localization, and gene expression.
Similarities and differences between high-throughput screening and high-content screening
Advantages of High-content Screening
- Diversity of screening results
- Multi-indicators and multi-targets interaction
- The detection volume does not increase with the increase of detection indicator
- Simple operation procedure and automation
- Information is obtained in cells, not microplates
Application of High-content Screening
- Used for cell cycle detection
- Used for cytotoxic detection
- Used for apoptosis detection
- Study on the mechanism of action for cell signal transduction
- Study on mechanism of action of protein-coupled receptors
Our Services
Cell function assays: cell proliferation, autophagy, apoptosis, migration, protein interaction.
Organelle and other micro structure detection: including mitochondria, lysosomes and lipid droplets, etc.
Fluorescence detection of organoids: fluorescence detection of cell balls, organoids and tissue sections.
High throughput drug screening: a large number of compounds can be screened by cells and organoids.
Data analysis
High-content screening (HCS) is a powerful tool for biological research, drug discovery, and toxicology screening, as it allows researchers to quickly and efficiently generate large amounts of data, identify new targets or compounds, and better understand complex biological processes. We provide several platforms and services to support your drug discovery process. For more detailed information, please feel free to contact us or directly sent us an inquiry.