Biotin Labeling

Biotin Labeling

BOC Sciences is proud to provide you high-quality biotin labeling using the most advanced and sophisticated technique. Our experts and chemists are very expert at biotin labeling to help you make progress in your work.

Introduction

Biotinylation is the process of covalently attaching biotin to proteins, nucleic acids or other molecules. Biotinylation is rapid, specific, and is unlikely to interfere with the natural function of molecules because of its small size (molecular weight = 244.31g/mol). The binding of biotin with streptavidin and avidin has high affinity, rapid opening rate and high specificity, and these interactions have been used to isolate biotinylated molecules of interest in many fields of biotechnology. In addition, a variety of biotin molecules can bind to the target protein, thus allowing multiple streptavidin, avidin or neutral avidin protein molecules to bind and improve the sensitivity of detecting the target protein.

Biotin LabelingFigure 1. The structural formula of biotin

Application of Biotin Labeling

  • It is an efficient and useful tool for many biomedical studies, such as various tracer immunoassays, immune-labeling, protein-labeling, molecular hybridization and labeling of nucleic acid probes.
  • Biotinylated proteins, such as biotinylated bovine serum albumin (BSA), are used as a coating on the pore surface of porous plates in solid phase determination.
  • Biotin tags can be used in conjunction with affinity chromatography columns with avidin (or antibiotic streptomycin or neutral avidin).

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Biotin Labeled Proteins

Biotin-labeled proteins are generated using internally developed single-point labeling and chemical labeling techniques, showing excellent biological activity and high detection sensitivity, and biotin does not affect the natural function of the protein itself.

Biotin labeled antibody is a technique for labeling biotin on specific antigens or antibody molecules. This enhanced amplification effect can show the characteristics and content of antigens or antibodies in the immune response system. Biotin labeled affinity purified antibodies are well suited for histochemical/cytochemical staining procedures and enzyme-amplified immunoassays.

Biotin-labeled nucleic acid probes and homologous sequences of nucleic acid molecules fixed on the filter membrane or in situ of tissue cells or chromosomes complement each other. By in situ hybridization, biotin-labeled specific gene probes can be used to directly detect tissue sections. Biotin-labeled nucleic acids can also be used for nucleic acid sequence analysis, gene isolation and purification, and identification of plant viruses and viroids.

Biotin-labeled peptides can be used for protein purification, detection, solidification, drug targeting, protein structure analysis, etc., and can be used as the main screening test for the identification of unknown protein-protein interactions.

Biotin can be coupled with oligonucleotides at the ends and internal locations of multiple sites, and can be used for the detection or separation of RNA and DNA because of its high affinity for streptavidin.

References

  1. Kim, D. I., Jensen, S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller biotin ligase for BioID proximity labeling. Molecular biology of the cell27(8), 1188-1196.
  2. Kim, D. I., & Roux, K. J. (2016). Filling the void: proximity-based labeling of proteins in living cells. Trends in cell biology26(11), 804-817.
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