Additional Conjugation Services

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Additional Conjugation Services

Bioconjugation involves covalently linking one biomolecule with another or with a synthetic molecule such as a dye, drug or a polymer, which endows biomolecule with desirable properties. There are several bioconjugation techniques varies and BOC Sciences can provide so many, such as biomolecule labeling, antibody conjugation, and additional conjugation services. Biomolecule labeling can be applied as signaling molecules, tools in molecular biology, and drugs in medicine. Antibody conjugation can improve or add valuable physicochemical, toxicological and pharmacological properties. With regard to some novel conjugates, the techniques of biomolecule labeling and antibody conjugation are not completely satisfactory. Additional conjugation services can be applied to make up for the deficiency.

Additional Conjugation Services Figure 1. The additional conjugation services that BOC Sciences can provide

Below is a list of our Additional Conjugation Services (include but not limited to the following):

Peptide bioconjugation refers to choosing certain peptide with site-specific and site-selective and flexible conjugation to cross-link with group of other molecules. Peptide bioconjugation can be applied to surface immobilized probes, which can facilitate diagnostic detection.

Protein can be conjugated to fluorescent dyes, drugs, polymers and other proteins, which has a broad range of applications. Protein bioconjugation methods are now extensively used in many chemistry, biology and nanotechnology research groups.

Enzymes, the superior biocatalyst, are involved in a range of biological reactions such as metabolism, DNA replication, gene expression, signal transduction, and so forth. After the conjugation between enzyme and molecular group, it can be applied not only in biotransformation, biosensors, and biomedical engineering fields but also in the production of pharmaceuticals and fine chemicals.

Liposome bioconjugation refers to the conjugation between liposome and oligonucleotides, oligosaccharides, peptides, antibodies or other molecules. After bioconjugation, liposome conjugates can also be used as vehicles to transport nucleic acids, peptides and proteins to cellular site in vivo. They also play an important role in macromolecular carrier, which can encapsulate cosmetic, drugs and fluorescent detection reagents.

Whether nanoparticles or macroparticles can be conjugated with biomolecules such as protein, enzymes, toxins, antibodies, drugs and so on, which have wide application in diagnosis. What’s more, nanoparticle and microparticles can also be applied in agglutination tests and assays, polymerase chain reaction (PCR) tests, particle capture ELISA methods, superparamagnetic-based assays and biosensors.

Site-specific ligation of polyethylene glycol (PEG) can be conjugated with peptide, protein, polymer micelles, antibodies, lectins, enzymes, drugs or nanoparticles, which has numerous promising applications in various fields. To prolong the circulation time of drug, PEGylation can be widely used via the enhanced permeability and retention effect, which provides a new hope for better treatment.

Nano-biological conjugated technology brings hope for the treatment of gliomas and other neurodegenerative diseases. Glioma is the deadliest type of cancer, and the options for treatment are very limited. In December 2010, scientists in the United States and Germany developed a multifunctional Nano-biological conjugated technology, which provided the ability to transport antisense oligodeoxynucleotides (AONs) across the blood-brain barrier (BBB) and the blood-brain tumor barrier (BTB), and targeted their payloads at tumors for receptor-regulated cell uptake, intracellular escape and release into the cytoplasm. Using this technique, the researchers demonstrated an inhibitory effect on the growth of intracranial tumors in a xenograft mouse model of human glioma.

Gold nanoparticles, which are small particles of gold with a diameter of 1 to 100 nm, have high electron density, dielectric properties and catalysis, and can bind to a variety of biological macromolecules without affecting their biological activity. Because spherical gold nanoparticles have strong adsorption function to protein, they can bind to staphylococcal protein A, immunoglobulin, toxin, glycoprotein, enzyme, antibiotic, hormone, bovine serum albumin and so on. Therefore, it has become a very useful tool in basic research and experiments.

BOC Sciences has the most professional bioconjugation team and we have competence and confidence to meet your demand for creating novel conjugates quickly and accurately. At BOC Sciences, our customers will be resourced with various kinds of synthetic and analytical instrumentations. Our experts will work with you to optimize the synthetic route and approaches. Additionally, according to your needs and the properties of chemical substances, a wide range of custom services are available in our laboratory.

References

  1. Chen, J., Saeki, F., Wiley, B. J., Cang, H., Cobb, M. J., Li, Z. Y., ... & Xia, Y. (2005). Gold nanocages: bioconjugation and their potential use as optical imaging contrast agents. Nano letters5(3), 473-477.
  2. Sperling, R. A., & Parak, W. J. (2010). Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences368(1915), 1333-1383.
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