Custom Oligonucleotides Synthesis

Oligonucleotides are a class of compounds composed of 15-50 base short chain nucleotides. Due to their unique mechanism of regulating the transcription and translation of disease genes at the RNA level, oligonucleotides are considered as the hot spot in the development of new generation of therapeutic drugs. Natural oligonucleotides are easily degraded in vivo and have low specificity, while synthetic oligonucleotides, as a new generation of gene medicines, avoid the shortcomings of natural oligonucleotides and can directly regulate gene expression, thereby play a role in the treatment of diseases. BOC Sciences provides oligonucleotide synthesis services via solid-phase synthesis.

Application of Oligonucleotides

Synthetic oligonucleotides have been used in PCR, reverse transcription, sequencing, gene editing, synthetic biology, cloning, SNP analysis, microarray, electrophoretic mobility analysis, and small RNA studies.

Classification of Oligonucleotides

Representative oligonucleotide drugs mainly include antisense oligonucleotide (ASO), small interfering RNA (siRNA), miRNAs, small activating RNA (saRNAs), aptamer, transcription factor decoy, ribozyme, etc.

Advantages of Oligonucleotides

Strong specificity: oligonucleotide drugs are designed according to the target RNA, so the target is clear and the target specificity is strong.

Simple design and short R&D cycle: The preclinical R&D of oligonucleotide drugs firstly involves gene sequencing, rational design for disease genes, and targeted gene silencing, so the R&D time can be saved to a large extent.

Abundant targets: oligonucleotide drugs can make breakthroughs for some special targets that have therapeutic effects on protein targets, which is expected to overcome the genetic diseases that have no drugs.

Advantages and disadvantages of oligonucleotides versus small molecule drugs (Phuc Tran, et al. 2022)

Our Services

• cGMP or research grade oligo synthesis
• Standard and custom oligo synthesis
• Provide a wide variety of oligonucleotide products
• Oligo internucleotide linkage and modifications
• Ribose modification

The modification of ribose sugar has a significant effect on the conformation of the sugar in the nucleotide, which determines the binding affinity of the oligonucleotide to its complementary strand and double-stranded structure.

• Base modification

By modifying the base of the nucleotide, a higher affinity for the target mRNA can be obtained, and the thermal stability of the duplex formed between the oligonucleotide and its target mRNA can also be increased, which can be greatly increased when performing mRNA silencing active.

• Bioconjugation

In addition to modifying oligonucleotides by changing their internal chemical structure, oligonucleotides can also be chemically modified by conjugating other molecules. This can affect the targeting and uptake of oligonucleotides at the tissue and cellular level, altering the metabolic kinetics of oligonucleotides.

Our Analytical method

• All oligonucleotides synthesis products (including miRNAs, siRNAs, ASO) are analyzed by mass spectrometry
• MALDI-TOFMS: for siRNA synthesis
• HPLC, PAGE, RNase Free HPLC and PAGE
• UPLC or CGE: for quality analysis when appropriate
• QC methods and documentation are available upon request

QC documentation

• Mass Spectrometry traces
• UPLC or CGE analysis
• Duplex or individual strand purity analysis
• Certificate of Analysis
• Endotoxin certification
• Custom QC documentation

BOC Sciences has years of professional and abundant experience which can offer a large variety of synthesis scales, modifications and purifications to satisfy your various needs.