Oligonucleotides are a class of compounds composed of 15-50 base short chain nucleotides. Due to their unique mechanism of regulating the transcription and translation of disease genes at the RNA level, oligonucleotides are considered as the hot spot in the development of new generation of therapeutic drugs. Natural oligonucleotides are easily degraded in vivo and have low specificity, while synthetic oligonucleotides, as a new generation of gene medicines, avoid the shortcomings of natural oligonucleotides and can directly regulate gene expression, thereby play a role in the treatment of diseases. BOC Sciences provides oligonucleotide synthesis services via solid-phase synthesis.
Synthetic oligonucleotides have been used in PCR, reverse transcription, sequencing, gene editing, synthetic biology, cloning, SNP analysis, microarray, electrophoretic mobility analysis, and small RNA studies.
Representative oligonucleotide drugs mainly include antisense oligonucleotide (ASO), small interfering RNA (siRNA), miRNAs, small activating RNA (saRNAs), aptamer, transcription factor decoy, ribozyme, etc.
Strong specificity: oligonucleotide drugs are designed according to the target RNA, so the target is clear and the target specificity is strong.
Simple design and short R&D cycle: The preclinical R&D of oligonucleotide drugs firstly involves gene sequencing, rational design for disease genes, and targeted gene silencing, so the R&D time can be saved to a large extent.
Abundant targets: oligonucleotide drugs can make breakthroughs for some special targets that have therapeutic effects on protein targets, which is expected to overcome the genetic diseases that have no drugs.
Advantages and disadvantages of oligonucleotides versus small molecule drugs (Phuc Tran, et al. 2022)
The modification of ribose sugar has a significant effect on the conformation of the sugar in the nucleotide, which determines the binding affinity of the oligonucleotide to its complementary strand and double-stranded structure.
By modifying the base of the nucleotide, a higher affinity for the target mRNA can be obtained, and the thermal stability of the duplex formed between the oligonucleotide and its target mRNA can also be increased, which can be greatly increased when performing mRNA silencing active.
In addition to modifying oligonucleotides by changing their internal chemical structure, oligonucleotides can also be chemically modified by conjugating other molecules. This can affect the targeting and uptake of oligonucleotides at the tissue and cellular level, altering the metabolic kinetics of oligonucleotides.
BOC Sciences has years of professional and abundant experience which can offer a large variety of synthesis scales, modifications and purifications to satisfy your various needs.