Purification refers to the separation of impurities in the mixture in order to improve its purity. As an important chemical method, it plays an important role not only in chemical research, but also in chemical production. And it usually includes physical methods and chemical methods. Custom Purification Services would provide customers all the compounds they require in their research. Highly purified products may be critical to your work, and this is particularly true for the generation of antibodies. Products preparations will often contain a number of substances from the production process that can adversely affect the quality of your research results.
Column chromatography is one of the important methods for separating and purifying organic compounds. The principle is that the components are separated from each other by repeated distribution according to the different distribution coefficients in the stationary phase and the flow phase. Because this method is usually carried out at room temperature, and is slow and time-consuming, especially in the preparation or separation of large dose groups, we need some flash column chromatography. For example, in the process of column chromatography, we can fill the gas to the elution liquid surface-pressure column chromatography, or use pump to form negative pressure in the lower end of the chromatographic column-negative pressure column chromatography, which accelerates the flow rate of the elution agent and shortens the operation time.
Preparative high performance liquid chromatography (HPLC) is a liquid chromatographic preparation method for high purity separation of samples on a high resolution, large inner diameter and high load separation column using high pressure and high flow liquid transport system. The product separated by this method is much better than the traditional preparation method in purity, recovery rate and separation efficiency, so it has been widely used in the field of drug research and production.
Resolution of enantiomers by HPLC and quantitative determination can be divided into chiral stationary phase method also known as direct method and chiral derivatization reagent method called indirect method, and chiral mobile phase additive method. Most of the previous studies and applications have been used for quality control or quantitative determination of enantiomeric purity of chiral drugs and chiral derivatization.
The solid crude products separated from organic synthesis often contain unreacted raw materials, by-products and impurities, which must be separated and purified. Recrystallization is one of the important and common separation methods for the separation and purification of pure solid compounds. Recrystallization is the process of dissolving a crystal in a solvent or melting and then re-crystallization from a solution or melt. Recrystallization can purify impure substances or separate mixed salts from each other. It is suitable for systems where the properties of products and impurities are quite different and the content of impurities in the products is less than 5%.