Fluorescent Dye Labeling

Fluorescent Dye Labeling

As a leading service provider in the field of drug discovery and research, BOC Sciences can provide you with high-quality fluorescent probes and multiple markers in the same molecule. Each labeled probe service is strictly monitored in accordance with our strict quality assurance and quality control standards to optimize the ratio of markers to biomolecules.

Introduction

Fluorescence labeling technology refers to the use of fluorescent substances using its fluorescence characteristics to provide the information of the object to be studied. Fluorescent labeling has the advantages of no radioactive pollution and easy to operate, which makes fluorescent markers more and more widely used in many research fields. The most commonly used fluorophores are fluorescein isothiocyanate (FITC), rhodamine derivatives (TRITC), coumarin and anthocyanin. These synthetic organic dyes are used to label biomolecules as proteins, peptides, antibodies, nucleic acids, bacteria or yeasts.

Application

  • The activity of the target protein was detected by using fluorescence-labeled peptides, and the developed high-throughput activity screening method was applied to drug screening and drug development of disease treatment target proteins (for example, various kinases, phosphatases, peptidases, etc.).
  • By fluorescence labeling biomolecules, researchers can use accurate and sensitive methods (such as imaging, flow cytometry, Western blotting, etc.) to detect specific components of complex biological complexes.
  • The life span of proteins was studied by using a hybridization system constructed from genetically modified small molecular organic fluorescent markers.

Below is a list of our fluorescent dye labeling (include but not limited to the following):

Fluorescent Labeling of Peptides

Labeling peptides with fluorescent dyes or other markers provides a powerful tool for the study of biological-related interactions, such as receptor-ligand binding, protein structure and enzyme activity.

Fluorescent labeling antibody technique is a chemical method to combine fluorescein with specific antibodies to form a fluorescein-protein conjugate (fluorescent labeled antibody). The fluorescein-protein conjugate still retains antibody activity and has the tracer effect of fluorescein. When it binds specifically to the corresponding antigen, it shows bright specific fluorescence with the help of fluorescence microscope.

As the preferred reagent for flow detection, fluorescent labeled protein can be detected by one-step staining because it is pre-labeled with fluorescein group, which not only simplifies the detection process and saves detection time, but also avoids the non-specific background caused by the use of secondary antibodies.

The fluorescent labeling of nucleic acids is usually carried out by enzymatic reaction, and the chemistry of organic fluorescent groups is introduced into primers or nucleosides of triphosphate. Fluorescence-labeled nucleotides have many advantages in the study of gene qualitative, quantitative, integration and expression. At present, it has been widely used in genetic disease diagnosis, virus infection analysis, prenatal diagnosis, tumor genetics, genome research and many other fields.

Polysaccharides are indispensable biological macromolecules in life science. Due to the lack of luminescent groups that are easy to detect, polysaccharides have been perplexing the further study of polysaccharides. Fluorescent substances have fluorescent groups, which are bound to sugar chain molecules by chemical synthesis, so that polysaccharides have fluorescent groups, so that we can detect them with common detection methods, so as to further understand the role of polysaccharides and study its mechanism.

In enzyme labeling, the DNA of genes and fluorescent proteins is first used to form DNA constructors. Heterozygous RNA+ fluorescence was formed after transcription. The object of interest connects to an enzyme that recognizes the heterozygous DNA. Fluorescein or biotin is usually used as a fluorophore.

Fluorescent dye-labeled oligonucleotides are important tools in biochemical and cellular research. At present, fluorescent oligonucleotides have been widely used in all major types of fluorescence imaging, including fluorescence resonance energy transfer (FRET). These labeled biomolecules are widely used in infectious disease diagnosis based on molecular beacons and other techniques. FRET oligonucleotides have also been used in cell analysis by fluorescence-related cell sorting (FACS) for in vivo or in vitro research and diagnostic purposes.

Magnetic nanoparticles have great advantages in biology. by combining magnetic nanoparticles with quantum dots, magnetic nanoparticles with superparamagnetism and fluorescence properties are obtained. as a result, the magnetic field control and fluorescence tracing of moving particles in molecules are realized, which can be used to target specific cells and tissues in vivo, or for the development of bioassay in vitro.

Common Fluorescein Information

Fluorescent Dye Labeling

References

  1. Kurutos, A., Ryzhova, O., Trusova, V., Tarabara, U., Gorbenko, G., Gadjev, N., & Deligeorgiev, T. (2016). Novel asymmetric monomethine cyanine dyes derived from sulfobetaine benzothiazolium moiety as potential fluorescent dyes for non-covalent labeling of DNA. Dyes and Pigments, 130, 122-128.
  2. Liu, J., & Cui, Z. (2020). Fluorescent labeling of proteins of interest in live cells: beyond fluorescent proteins. Bioconjugate chemistry, 31(6), 1587-1595.
  3. Toseland, C. P. (2013). Fluorescent labeling and modification of proteins. Journal of chemical biology, 6(3), 85-95.
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