Some studies show that various enzymes can be attached to antibodies and proteins using different coupling chemistries, among which the most common enzymes are horseradish peroxidase (HRP) and alkaline phosphatase (AP). Antibody-enzyme conjugates are the most extensive application of antibody conjugation. The conjugation of enzymes to antibodies is the formation of a stable, covalent linkage between an antigen-specific monoclonal or polyclonal antibody and enzyme. Antibody-enzyme conjugate can keep all the immunoreactivity and enzyme activity of its components. In this system, antibodies are used as carriers for enzymes to tumor cell surfaces, and the various abilities of enzymes are chosen to convert relatively noncytotoxic drug precursors (pro-drugs) into active anticancer drugs. The application area of antibody-enzyme conjugate includes Enzyme-linked immunosorbent assays (ELISAs), Blotting techniques, In situ hybridization, Cytochemistry and Histochemistry detection system. Antibody-enzyme conjugation has been designed as a new approach for disease therapy, especially for cancer therapy.
With years of experience in conjugating of reagents and antibodies, BOC Sciences offers you high quality, efficient and cost-saving antibody conjugation services. Our chemists can choose appropriate reagents and reaction to create antibody-enzyme conjugates according to the different functional groups of enzymes and antibody molecules available to meet your requirement. The antibody-enzyme conjugates we offer include HRP-Antibody conjugate, AP-Antibody conjugate and β-gal-Antibody conjugate.
Antibodies conjugated with horseradish peroxidase HRP are one of the most widely used bioreagents in biological science. HRP can be either directly conjugated to the antibody of interest or linked to a secondary antibody of interest. Antibody-HRP conjugate may be used to detect a small amount of a specific protein in a western blot, in which the antibody provides the specificity to locate the protein of interest, and the HRP enzyme, in the presence of a substrate, produces a detectable signal.
Compared with HRP, alkaline phosphatase (AP) has a lower catalytic rate, greater stability and maintains linear reaction kinetics for much longer. AP-conjugated antibody is prepared by crosslinking the appropriate primary antibody with alkaline phosphatase using glutaraldehyde. AP-antibody conjugate is commonly applied in immunoassays.
β-galactosidase is one of the most widely used enzymes in molecular biology since it has a high turnover rate and can be used with substrates yielding both soluble and insoluble products. β-galactosidase antibody conjugates are excellent choices for photometric and electrochemical enzyme-multiplied assay techniques (EMATs). β-galactosidase antibody is prepared from monospecific antiserum through a delipidation and defibrination.
BOC Sciences has successfully developed varieties of antibody-enzyme conjugates. Please contact us for more on custom antibody-enzyme conjugation services. We expect to hearing from you.