BOC Scientific provides a variety of resolution methods and detailed analysis data to analyze and screen samples for customers worldwide. We will support the best comprehensive solution for chiral analysis and separation.
Chirality exists in many substances in nature. For example, naturally occurring sugars as well as nucleic acids, starch, cellulose, proteins, and amino acids are all chiral compounds. Chiral analysis and separation refer to the analysis of chiral molecules that are mixed together by a range of modern analytical devices and methods, including crystallization resolution, chemical resolution, enzymatic resolution, high performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), capillary electrophoresis (CE), sensor methods, and spectrometry.
The significance of chiral separation is its role in chemical drugs that are used for human disease treatment and in fine chemicals such as insecticides, fungicides, insect sex pheromones, plant growth regulators, food additives, spices, etc. One part has one to two chiral centers. Many studies have shown that a single-configuration chiral molecule is usually a stereoisomer with utility value, but its mirror image molecule is useless or even harmful. Therefore, it is necessary to study the physicochemical properties of chiral substances that are enantiomers to each other, and obtain a single chiral enantiomer that is beneficial to the human body and the environment.
The separation and analysis methods of chiral substances include crystallization resolution, chemical resolution, kinetic resolution and chromatographic resolution that are often combined with centrifuge separation. Common chromatographic resolution methods include capillary electrophoresis (CE), high performance liquid chromatography (HPLC), gas chromatography (GC), thin layer chromatography (TLC), and supercritical fluid chromatography (SFC). Chromatography has become the main tool for current chiral analysis and separation. Taking HPLC as an example, it has a wide range of applications and strong separation capability, and it has become one of the preferred technology platforms for the separation of chiral compounds. SFC, another example, is a new chromatographic technique. SFC can be used as an alternative to normal phase HPLC and is often used for the separation of chiral compounds. SFC can be used as a complementary technology to HPLC and GC.
The identification and quantification of compounds are determined by chromatograms, that is, determine what each chromatographic peak represents, and then further determine the composition of the sample mixture composed of these components. Generally, the x-axis represents the retention time, and the y-axis represents the absorption intensity measured by the UV detector. The retention time is related to the structure and properties of the components, and is a qualitative parameter that can be used for compound identification. The methods for identifying compounds by HPLC and SFC are basically the same
Enantiomeric separation is a key step in the development of new chiral drugs. The enantiomeric compounds have exactly the same physical and chemical properties except for the opposite direction of polarized light deflection, so it is difficult to separate them. Traditional methods (crystal resolution method, enzyme resolution method, etc.) have great limitations. HPLC has become the most widely used method for enantiomeric separation. The method for the purification of enantiomers by HPLC and SFC is generally referred to as the continuous adsorption and desorption of compounds between the stationary phase (the column) and the mobile phase so that different compounds can be separated. Because different compounds have different forces between the two (stationary phase and mobile phase). Some chiral substances and spatial isomers have almost the same polarity, so they are difficult to separate. Therefore, special chiral columns are needed to separate them for purify.
We provide regulatory-driven analysis to support your regulatory plans, such as new chemical notification research or drug development requirements. BOC Sciences has state-of-the-art HPLC, GC, CE, and SFC equipment, as well as a dedicated team of experts who can customize the chiral analysis and separation services for you.