Chiral Analysis and Separation

Chiral Analysis and Separation

Chirality exists in many substances in nature. For example, naturally occurring sugars as well as nucleic acids, starch, cellulose, proteins, and amino acids are all chiral compounds. Chiral analysis and separation refers to the analysis of chiral molecules that are mixed together by a range of modern analytical devices and methods, including high performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), capillary electrophoresis (CE), sensor methods, and spectrometry.

Application of Chiral Analysis and Separation

The significance of chiral separation is its role in chemical drugs that are used for human disease treatment and in fine chemicals such as insecticides, fungicides, insect sex pheromones, plant growth regulators, food additives, spices, etc. One part has one to two chiral centers. Many studies have shown that single-configuration chiral molecule is usually a stereoisomer that has utility value, but its mirror image molecule is useless or even harmful. Therefore, it is necessary to study the physicochemical properties of chiral substances that are enantiomers to each other, and obtain a single chiral enantiomer that is beneficial to the human body and the environment.

BOC Sciences has advanced equipment and first-class technology to provide chiral analysis and separation services.

Chromatographic techniques commonly used for chiral separations include: TLC, GC, CE, SFC, and HPLC. Chromatography has become the main tool for current chiral analysis and separation. Taking HPLC as an example, it has a wide range of applications and strong separation capability, and it has become one of the preferred technology platforms for the separation of chiral compounds. SFC, another example, is a new chromatographic technique. It can separate and analyze objects that gas and liquid chromatography cannot.

The method for identifying compounds by HPLC and SFC is basically the same, and it is to determine what each chromatogram peak represents, and thus determine the composition of the sample mixture made up of these components.

The method for the purification of enantiomers by high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) is generally referred to as the continuous adsorption and desorption of compounds between the stationary phase (the column) and the mobile phase so that different compounds can be separated. Because different compounds have different forces between the two (stationary phase and mobile phase). Some chiral substances and spatial isomers have almost the same polarity, so they are difficult to separate. Therefore, special chiral columns are needed to separate them for purify.

Our Advantages

  • Ship compounds in milligrams to kilograms. Typically, smaller samples are processed in a few days, and the process we develop can be extended as your program needs to be upgraded.
  • A chromatographic solution that suits your schedule and budget.
  • Normal phase or SFC for rapid analysis and process monitoring.
  • Validation-prepare for bulk publishing and stability.
  • Inverse biological analysis.

We provide regulatory-driven analysis to support your regulatory plans, such as new chemical notification research or drug development requirements. BOC Sciences has state-of-the-art HPLC, GC, CE, and SFC equipment, as well as a dedicated team of experts who can customize the chiral analysis and separation services for you.

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