Biomolecule Labeling

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Biomolecule Labeling

Biomolecule labeling generally refers to cross-linking or modification in any form, whose purpose is to conjugate a chemical group with target molecules through single, bifunctional or heterofunctional cross linkers to aid in detection. The chemical group can be fluorescent, biotin, haptens, nanoparticles or other target functional group and the target molecules can be proteins, nucleic acids and other biomolecules.

Application of biomolecule labeling

Biomolecule labeling has advantage of signal amplification and biologic specificity, which can easily detect small specific biomolecule or change the native properties and function of target biomolecule. All in all, biomolecule labeling plays an important role in the elucidation of complicated biological processes and is highly useful in the construction of various small-molecule probes to explore complex biological systems.

Biomolecule Labeling Figure 1. Labeled avidin- biotin technique

Below is a list of our Biomolecule Labeling Services (include but not limited to the following):

Biotin labeling uses biotin to label nucleic acid, protein, peptide and other biomolecules, which has the advantage of high affinity, stability, accuracy, convenience and multistage amplification. What’s more, it is harmless to people when compared with radioactive isotope. Therefore, it is an efficient and useful tool for many biomedical studies, such as various tracer immunoassays, immune-labeling, protein-labeling, molecular hybridization and labeling of nucleic acid probes.

Fluorescent dye labeling uses a variety of fluorescent dyes to label biomolecules, which has high quantum yield of fluorescence, high environmental stability, better cell penetration and intracellular solubility and minimal toxicity to the cell tissue of an organism. Therefore, it can be applied in many fields such as DNA sequencing, detection of cell, pharmacology, toxicological analysis, drug target design, and clinical diagnoses.

Enzyme labeling is a new technology, which is developed on the basis of labelled antibody (fluorescent dye labeling, isotope labeling) and histochemistry. When compared with immunofluorescence, radioimmunoassay and ferritin labeling, enzyme labeling has the advantage of high sensitivity, specificity, and diagnosis rapidly, with no special equipment needed.

Custom-made compounds labeling refers to using supplied compound having target functional group to conjugate biomolecule. First of all, BOC Sciences can synthesize compounds having target functional group for what you want or you can provide us with special compounds you have. Then we choose the best protocol to achieve the goal of labeling.

BOC Sciences is specialized in the biomolecule labeling services, providing a confidential and efficient service at competitive prices. Every step of peptide synthesis is subject to BOC Sciences’ stringent quality control. We can provide varieties of biomolecule labeling services you want. Our chemistry team are the most professional and we have competence and confidence to meet your demand for biomolecule labeling that can be quickly and accurately. Glad to hear your comments and look forward to cooperating with you.

References

  1. Ackerson, C. J., Powell, R. D., & Hainfeld, J. F. (2010). Site-specific biomolecule labeling with gold clusters. In Methods in enzymology (Vol. 481, pp. 195-230). Academic Press.
  2. Morales, A. R., Yanez, C. O., Schafer-Hales, K. J., Marcus, A. I., & Belfield, K. D. (2009). Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe. Bioconjugate chemistry20(10), 1992-2000.
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