Oxidized Lipids

What are Oxidized Lipids?

Oxidized lipids are a series of oxidized metabolites produced by the auto-oxidation of polyunsaturated fatty acids (straight-chain fatty acids with two or more double bonds and a carbon chain length of 18 to 22 carbon atoms) or by the action of specific enzymes. Polyunsaturated fatty acids include arachidonic acid, linoleic acid, α-linolenic acid, DHA, EPA, DGLA, and specific enzymes include COX, LOX, and CYP450. Oxidized lipids play important roles in inflammatory response, immune defense, endocrine regulation, and oxidative stress.

Fig 1. Structures of oxidized lipids. (Ouchi et al., 2019)

Oxidized Lipid Synthesis Services from BOC Sciences

Before proceeding with the preparation of oxidized lipids, the design of the composition and structure of the oxidized lipids needs to be considered. Firstly, the unsaturated fatty acid content needs to be regulated and suitable oxidizing agents need to be selected. Secondly, specific functional groups or functional groups need to be introduced into the molecular structure of the lipid according to the customer's needs, which can be hydroxyl groups, thiol groups, etc. Finally, the packaging system of the lipid needs to be considered, and suitable carriers or microcapsules etc. need to be selected for better application in various fields.

• Preparation of oxidized lipids

According to the needs of customers, the preparation of oxidized lipids can be carried out in the following aspects.

Firstly, oxidized lipids are oxidized by selecting oxidants method. These oxidizing agents can be hydrogen peroxide, benzoyl peroxide, butanone peroxide and so on. This type of reaction is suitable for room temperature or heating conditions.

Secondly, oxidation of lipids can also be carried out under high temperature conditions, where the medium of oxidation is thermal oxygen, and oxidized lipids are prepared by high temperature catalysis.

Finally, photo-oxidation is also a way to prepare oxidized lipids. The target product can be prepared under UV light irradiation using oxygen as the oxidizing agent.

• Customized oxidized lipids are available

Omega-3 and Omega-6 (In polyunsaturated fatty acid molecules, those with the double bond furthest from the carboxyl group on the penultimate carbon atom are called omega-3; those with the double bond furthest from the carboxyl group on the sixth carbon atom are called omega-6).

• Detection of Oxidized Lipids

Detection of oxidized lipids is mainly performed by targeted mass spectrometry. BOC Sciences combined two special algorithms to establish a prediction method for secondary mass spectrometry fragmentation of lipid oxidation products and generated a library of predicted MS/MS of oxygenated oxidation products. The oxidized lipids were analyzed by ultra performance liquid chromatography tandem high resolution mass spectrometry. The products of lipid oxidation were detected by the identification of the predicted MS/MS1 library. By matching the characteristic fragments of oxidized lipids, the predictive MS/MS library is able to identify the oxidation products quickly and accurately, which greatly improves the analysis efficiency.

Application of Oxidized Lipids

• Disease research: for cardiovascular, tumor, immune, endocrine, renal disease, digestive system, nervous system and other types of disease research.
• Drug target research: by inhibiting TXA2 or PGI2 in oxidized lipids, it plays a corresponding role in anti-platelet aggregation or anti-inflammatory effects.
• Disease biomarker screening: screening disease biomarkers by studying the differences in oxidized lipids between groups, which can be used for disease diagnosis, typing, prognosis and other studies.
• Healthcare function research of healthcare products: many healthcare products contain polyunsaturated fatty acids such as DHA, whose oxidized metabolites have the functions of regulating blood pressure, inhibiting inflammation, and inhibiting angiogenesis.

BOC Sciences provides professional oxidized lipid synthesis services and absolute quantitative detection of oxidized lipids by double validation of internal & external standards. If you are interested in our services or the service you want is not in the list, feel free to contact us and we will customize a professional solution for you.