Technologies for Chiral Analysis and Separation

As one of the most key elements for any pharmaceutical development program, analytic method development, validation...

Technologies for Chiral Analysis and Separation

The numerous studies have been focused on chiral analysis and the separation of chiral compounds resulting in various approaches to chiral recognition, analysis and separation, including capillary electrophoresis, high performance liquid chromatography, thin-layer chromatography, supercritical fluid chromatography, nuclear magnetic resonance, chiral ligand-exchange chromatography, circular dichroism, diastereomeric crystallization, and molecular imprinting techniques. The common chiral separation technologies could be classified into several subtypes as follow´╝Ü

Sub- and Supercritical Fluid Chromatography

Polysaccharides are naturally occurring polymers having the capability for chiral separations because of their asymmetric structures. The polysaccharide chiral selectors have a wide range of applications in subcritical fluid chromatography (sub-SFC) and supercritical fluid chromatography (SFC). Unlike in HPLC, temperature and pressure are important parameters in SFC and, hence, they have been used for the optimization of chiral separation in the modality of liquid chromatography.

Capillary electrochromatography (CEC) is considered to be a good technique for chiral separations as it works on the principles of both liquid chromatography and capillary electrophoresis. Chiral separations in CEC have been carried out by using capillaries packed with polysaccharide chiral selectors. The packed capillaries were prepared by a slurry packing method

Thin layer chromatography (TLC) is a chromatographic technique for the separation of non-volatile mixtures. Thin layer chromatography is carried out on a piece of glass, plastic or aluminum foil coated with a thin layer of adsorbent material, usually silica gel, alumina or cellulose. It is a rapid and simple method for the analysis of organic mixtures.

The chromatographic techniques have frequently been used for enantiomeric resolution using polysaccharide chiral selectors, but the inherent limited resolving power and efficiencies, laminar flow and mass transfer terms attracted workers towards capillary electrophoresis (CE) in the chiral separation area. At present, CE is a versatile technique of high speed, sensitivity and low running costs, which is a major trend in analytical science, and numerous publications have been appeared on the chiral resolution of a wide variety of race- mates.

Our Services

As discussed above, there are different variations of the technology for chiral analysis and separation. BOC Sciences has all kind of testing instruments and technologies. Our experienced scientists will tailor the most appropriate methods and 100% guaranteed service for customers. Typical services include:

  • Chiral separation service
  • Chiral Analysis report
  • Technical consultation


  1. Kim, J. M., Chang, S. M., He, X. K., & Kim, W. S. (2012). Development of real-time sensitive chiral analysis technique using quartz crystal analyzer. Sensors and Actuators B: Chemical, 171, 478-485.
  2. Guo, H. S., Kim, J. M., Kim, S. J., Chang, S. M., & Kim, W. S. (2008). Versatile method for chiral recognition by the quartz crystal microbalance: chiral mandelic acid as the detection model. Langmuir, 25(2), 648-652.
  3. Subramanian, G. (Ed.). (2008). Chiral separation techniques: a practical approach. John Wiley & Sons.
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