Stable Isotope Labeling

Stable Isotope Labeling

What is Stable Isotope Labeling?

The practice of stable isotope labeling is a widely utilized method across the scientific disciplines, spanning chemistry, biology, and environmental science, among others, in order to effectively monitor the migration of atoms or molecules within a given system. The process itself involves the substitution of one or more atoms present within a molecule with a stable isotope of the identical element, wherein the likes of carbon-12 is supplanted by carbon-13, for example.

By and large, these stable isotopes possess equivalent chemical properties to their non-labeled counterparts, but their mass deviates slightly, thus making them perceptible and hence, capable of being tracked with the aid of specialized analytical methods like mass spectrometry.

Application of Stable Isotope Labeling in pharm

Metabolism studies: Tracing the metabolic fate of drugs in the body. Researchers can track its breakdown and the formation of metabolites using mass spectrometry or nuclear magnetic resonance (NMR) spectroscopy by labeling a drug with a stable isotope. This information is critical for understanding the safety and efficacy of drugs.

Bioavailability studies: Determining the bioavailability of drugs. Researchers can quantify the amount of drug that is absorbed and the rate at which it is eliminated from the body by labeling a drug with a stable isotope.

Pharmacokinetics studies: Studying the pharmacokinetics of drugs. Researchers can determine the rate of absorption, distribution, metabolism, and excretion of a drug by labeling a drug with a stable isotope.

Drug-drug interactions: Studying drug-drug interactions. Researchers can determine if the second drug affects the pharmacokinetics or metabolism of the labeled drug by labeling one drug with a stable isotope and administering it with another drug.

Methods of Stable Isotope Labeling

Metabolic labeling: In this method, cells or organisms are grown in a culture medium that contains a labeled nutrient, such as a stable isotope of an amino acid. The labeled nutrient is incorporated into proteins, nucleic acids, or other biomolecules, which can then be tracked and studied.

Chemical labeling: Involving chemically modifying a molecule or compound with a stable isotope-labeled group, such as a methyl group or a carbonyl group. This method is often used in proteomics and metabolomics research to study the function and metabolism of specific molecules.

Isotope dilution: Determining the concentration of a particular molecule or compound in a sample. A known amount of a stable isotope-labeled form of the molecule is added to the sample, and the ratio of labeled to non-labeled forms is measured. This allows researchers to calculate the concentration of the molecule in the original sample.

Available isotopes

  • Carbon-13
  • Nitrogen-15
  • Oxygen-18
  • Sulfur-34
  • Deuterium
  • Other isotopes (please inquire)

Additional services

  • Storage
  • Re-analysis
  • Repurification
  • Stability studies

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