BOC Sciences offers comprehensive targeted kinase inhibitor activity screening services to support drug discovery and development. Leveraging advanced screening platforms and a diverse kinase library, we provide high-throughput profiling of kinase inhibitors to evaluate their potency, selectivity, and mechanism of action. Our services cover a broad range of kinase targets, including tyrosine kinases, serine/threonine kinases, and lipid kinases, enabling precise identification of lead compounds or optimization of existing candidates. With state-of-the-art technologies, such as fluorescence-based assays, radioisotope detection, and biochemical/cellular screening models, we deliver reliable and reproducible data. Our expert team tailors screening workflows to meet specific research needs, ensuring rapid turnaround times and actionable insights to accelerate your kinase-related therapeutic projects.
The structural similarities across kinase families make the identification of highly selective kinase inhibitors difficult. How do off-target effects affect drug efficacy and safety profiles? Given the extensive variety of kinase isoforms and complex signaling networks, how can you ensure accurate target validation while reducing both false positives and false negatives? Standard screening methods frequently have inadequate sensitivity together with low throughput which increases costs and postpones research timelines. The inadequacy of analytical tools limits the ability to derive mechanistic insights from complex datasets. Every stage of kinase inhibitor development encounters numerous obstacles. To surpass these challenges researchers must implement advanced screening technologies and develop customized solutions that boost discovery speed and enhance therapeutic results.
We offer various flexible detection methods, including biochemical assays, cell models, and functional assays, to meet diverse research needs.
Equipped with state-of-the-art facilities and technical platforms, ensuring efficient, precise testing and reliable experimental data.
Supports a wide range of kinase targets, covering various kinase subtypes, mutants, and disease-related signaling pathways.
From demand analysis and experimental design to report delivery, we provide tailored services to meet the unique research needs of our clients.
We provide detailed experimental reports, including IC50 curves, screening results, structure-activity relationship analysis, and more, to help clients accurately interpret data.
Our team of seasoned experts offers professional technical support and consultation, ensuring the accuracy and reliability of experimental results.
BOC Sciences provides comprehensive kinase testing programs, offering target validation, inhibitor screening, activity analysis, and mechanism studies to support efficient drug discovery across oncology, immunology, and metabolic diseases.
Determines the concentration at which a compound reduces kinase activity by 50%, serving as a key parameter for potency evaluation.
Measures the binding affinity of a compound competing with ATP for kinase interaction, offering insight into inhibition mechanisms and strength.
Assesses the inhibitory effect of compounds across a panel of over 500 kinases, helping reveal broad-spectrum activity and potential off-target effects.
Compares IC50 values between wild-type and mutant kinases to evaluate compound sensitivity, resistance profiles, and mutation-driven efficacy changes.
Quantifies the fold change in phosphorylation signals after compound treatment, providing insights into pathway modulation and biological activity shifts.
Analyzes the real-time binding (Kon) and dissociation (Koff) rates between compounds and kinases to define kinetic behavior and interaction stability.
Indicates the equilibrium binding affinity between a compound and kinase, with lower KD values suggesting stronger, more stable molecular interactions.
Identifies the number of non-target kinases inhibited above 30%, helping evaluate compound selectivity and potential off-target safety risks.
BOC Sciences possesses cutting-edge instrumentation platforms that enable the accurate execution of multiple kinase detection techniques. The availability of top-tier liquid scintillation counters along with fluorescence polarization readers and FRET detection systems enables us to achieve both high sensitivity and precise accuracy in experimental data. Our mass spectrometers along with chemiluminescence imaging systems and automated sample processing systems deliver enhanced detection stability and efficiency for complex scientific research applications. Our advanced instrumentation platforms enable us to provide dependable and effective kinase activity detection services that propel drug development and biological research advancements for researchers and pharmaceutical companies.
| LC-MS/MS system | Fluorescence polarization reader | Nano-flow liquid chromatography system |
| ELISA reader | Multifunction microplate reader | Automated sample processing system |
| Liquid scintillation counter | FRET detection system | Chemiluminescence imaging system |
| Gamma counter | Liquid handling workstation | High-sensitivity plate reader |
BOC Sciences provides numerous kinase assay options specifically designed to fulfill diverse research objectives and experimental conditions. We deliver adaptable and effective tailored solutions for each target type and sample characteristic through highly sensitive radioactive isotope assays for precise quantification or fluorescence-based methods forhigh-throughput screening which support precise kinase research and drug discovery.
This method employs ATP labeled with radioactive isotopes (such as 32P or 33P). When a kinase transfers the phosphate group to a substrate, the resulting radioactivity can be measured to determine enzyme activity. It offers high sensitivity and is suitable for analyzing classical kinases such as MAPK, CDK, and PKA. It is ideal for quantifying kinase-substrate interactions and studying phosphorylation mechanisms in depth.
This technique measures the change in rotational diffusion of fluorescently labeled small-molecule substrates after phosphorylation, which alters the fluorescence polarization value. It is well-suited for high-throughput screening and commonly used in the study of kinases like PI3K, JAK, and BTK. The method helps in identifying kinase inhibitors and characterizing their effects during early drug discovery.
FRET (Fluorescence Resonance Energy Transfer) assays detect phosphorylation-induced changes in spatial proximity between donor and acceptor fluorophores on substrates, which affect energy transfer efficiency. This method is excellent for monitoring kinase activity in live cells and is often used to study EGFR, SRC, and AKT. It provides dynamic insights into kinase signaling within intracellular environments and is valuable for real-time interaction studies.
This assay utilizes chemiluminescent substrates that emit light upon reacting with phosphorylation products, with signal intensity reflecting kinase activity. It is suitable for detecting kinases with low activity levels, including GSK-3β, Chk1, and IRE1. This technique is widely used in drug development and diagnostics, especially when analyzing disease-related kinase signaling such as HER2 or BCR-ABL.
Assay kits are based on ELISA or colorimetric reactions that detect phosphorylated substrates via specific antibodies, generating quantifiable color changes. They provide a convenient solution for routine detection of kinases such as ERK, JNK, AMPK, and mTOR. These kits are widely used in biological research and pharmacological screening involving cell lysates and tissue extracts.
Mass spectrometry identifies and quantifies phosphorylated peptides, enabling precise mapping of kinase modification sites and activity levels. It is suitable for profiling complex kinase-substrate networks and discovering new targets for kinases like CK2, ATM, and LKB1. This method supports phosphoproteomics and advanced analysis of cell signaling pathways under physiological or stressed conditions.
Requirement Understanding
Communicate with the client to understand their research goals and needs, including selecting specific kinase targets, mutants, or disease-related signaling pathways, ensuring the test content aligns with the client's research direction.
Experimental Design
Develop a detailed experimental design plan based on the requirements, selecting appropriate detection methods (such as biochemical or cellular models), and determining the necessary compound information (such as concentration range and expected mechanisms).
Experimental System Construction
Prepare the required kinases, substrates, and other experimental reagents, ensuring the recombinant expression and purification of kinases, and optimize experimental conditions to ensure system stability and achieve the best signal-to-noise ratio.
Experimental Testing
Conduct dose-response analysis to test the inhibitory effects of compounds, obtain key parameters like IC50 values, and further optimize test conditions based on the results.
Data Collection
Collect and organize experimental data, including compound activity data, selectivity data, off-target effects, etc., and perform comprehensive analysis of all relevant data.
Report Delivery
Analyze the experimental results and generate a detailed test report, including IC50 curves, kinase screening results, structure-activity relationship tables, etc., helping the client understand the results and providing improvement recommendations.
BOC Sciences offers comprehensive kinase profiling services across multiple disease areas, delivering high-precision data to drive innovative drug development. We support target research in oncology, metabolic disorders, neuropsychiatric diseases, and immunomodulation, covering over 500 kinase targets—including wild-type, mutant, and disease-relevant isoforms. Through rigorous experimental design, multi-level analytical models (biochemical, cellular, and functional validation), and a multidimensional quality control system, we ensure the accuracy and reproducibility of activity screening, selectivity assessment, and mechanistic studies. Whether for single-target validation or complex signaling network analysis, we provide customized solutions to accelerate the transition from early discovery to preclinical research, offering reliable and efficient kinase drug development support for global research teams and pharmaceutical companies.
| Target Category | Kinase Targets | Application Scenarios |
| Targeted Cancer Therapies | EGFR, ALK, BRAF, HER2, VEGFR, MET, ROS1 and other kinases involved in tumor proliferation, angiogenesis, and metastasis. | Activity validation of small molecule kinase inhibitors, resistance mechanism studies, and optimization of combination therapies. |
| Immunomodulatory Drugs | JAK family (JAK1/2/3, TYK2), BTK, SYK and other kinases that regulate immune cell signaling pathways. | Target evaluation for autoimmune diseases (e.g., rheumatoid arthritis, psoriasis) and hematologic malignancies. |
| Anti-inflammatory & Anti-fibrotic Drugs | p38 MAPK, ROCK, TAK1, and kinases related to TLR pathways. | Mechanism investigation and off-target effect assessment of drugs for chronic inflammatory diseases such as pulmonary fibrosis and liver cirrhosis. |
| Neurological Disease Drugs | CDK5, GSK-3β, LRRK2 and other kinases associated with neurodegenerative diseases (e.g., Alzheimer's, Parkinson's). | Screening of agents targeting abnormal phosphorylation processes and studies on neuroprotective mechanisms. |
| Anti-infective Drugs | Host or pathogen kinases (e.g., HCV NS5B, HIV integrase co-factors). | Development of inhibitors targeting key kinases involved in viral replication and investigation of host-pathogen interactions. |
| Metabolic Disease Drugs | AMPK, mTOR/PI3K-Akt pathway, JNK, ROCK—key kinases regulating energy metabolism, lipid synthesis/degradation, and insulin sensitivity. | Screening of small molecule agonists/inhibitors (e.g., AMPK activators, mTOR inhibitors); activity validation in adipose or liver-specific models; mechanistic studies of combination therapies for metabolic syndromes such as diabetes and NAFLD. |
What is a kinase assay?
A kinase assay is a laboratory test used to measure the activity of kinases, which are enzymes that catalyze the transfer of phosphate groups from ATP to specific substrates. This assay helps evaluate the enzyme's activity, identify potential inhibitors, and investigate kinase-related signaling pathways. Kinase assays are commonly used in drug discovery, cancer research, and other biochemical studies.
What is creatine kinase assay?
A creatine kinase (CK) assay serves as a diagnostic tool that quantifies the functional levels of creatine kinase enzyme present within cardiac, cerebral, and muscular tissues. The enzyme creatine kinase facilitates the transformation process which turns creatine and ATP into phosphocreatine and ADP.
What is the purpose of kinases?
The purpose of kinases is to regulate various cellular processes by adding phosphate groups to proteins, lipids, or other molecules. This phosphorylation modifies the target's activity, interactions, localization, or stability, thus influencing cellular functions such as metabolism, signal transduction, gene expression, and cell division. Kinases play a crucial role in regulating many physiological and pathological processes.
What is the main function of a kinase?
The main function of a kinase is to catalyze the transfer of phosphate groups from ATP to specific substrates, a process known as phosphorylation. This modification can activate or deactivate enzymes and proteins, control cell signaling pathways, and regulate various cellular functions such as growth, differentiation, and apoptosis.
