BOC Sciences offers professional Cell Proliferation and Activity Study services, encompassing a variety of assay methods and experimental models to assess the impact of compounds on cell growth, viability, and functional status. Leveraging advanced experimental platforms and an experienced technical team, BOC Sciences provides customized research solutions for clients, ensuring the accuracy and reliability of experimental data. Our services comprehensively support drug screening and mechanism research, accelerating the progress of scientific studies.
Cell proliferation and activity studies present challenges such as complex experimental techniques, high equipment and reagent costs, large data volumes requiring advanced analysis, lack of specificity in cell models, and high demands for dynamic monitoring. These issues can lead to data discrepancies, extended timelines, and high expenses for small research groups. Relying on professional testing companies like BOC Sciences offers standardized procedures, advanced equipment, and expert teams, ensuring reproducibility, reducing costs, and providing precise data analysis. Outsourcing can accelerate research, ensure compliance, and provide reliable, high-quality results, enabling researchers to focus on their core objectives.
Offer various testing methods to meet different experimental needs, ensuring a comprehensive evaluation of cell activity.
Utilize high-throughput platforms and automation technology to improve experiment efficiency and data consistency.
Provide personalized experimental design and custom services based on client needs to ensure research objectives are achieved.
Deliver precise data analysis with easy-to-understand reports to help clients quickly interpret results.
Support testing for a variety of cell lines, catering to research needs across different fields.
Use cutting-edge experimental equipment and technology to ensure high efficiency and accuracy in testing.
BOC Sciences offers comprehensive cell proliferation and activity testing services, including proliferation analysis, viability assessment, apoptosis detection, and signaling pathway evaluation to accelerate drug development across oncology, inflammation, and metabolic diseases.
Measures the change in cell number over time to assess proliferation dynamics under different conditions.
Determines the percentage of living cells using assays such as MTT, CCK-8, or ATP detection, reflecting treatment toxicity and cell health.
Analyzes the distribution of cells across G0/G1, S, and G2/M phases to evaluate proliferation control and arrest mechanisms.
Quantifies early and late apoptosis using Annexin V/PI staining, offering insights into cell death pathways induced by compounds.
Assesses DNA replication using EdU or BrdU incorporation assays, indicating active cell division and proliferation rates.
Evaluates cell motility and invasiveness through scratch wound healing assays and Transwell chamber assays.
Measures activation changes in critical pathways such as MAPK and PI3K/Akt, providing mechanistic insights into cellular responses.
Detects key markers like Ki-67 and PCNA via immunostaining or Western blot to monitor cell proliferation and cycle progression.
BOC Sciences offers a diverse and expandable portfolio of cell models, including but not limited to tumor cell lines, primary cells, stem cells, organoids, and immune cells, to support comprehensive cell proliferation and activity studies. Our standardized platforms and expert techniques enable flexible model selection and provide reliable tools for drug screening, toxicity evaluation, and mechanism exploration across a broad range of biomedical research needs.
| Model Type | Representative Examples |
| Tumor Cell Lines | HeLa (cervical cancer), MCF-7 (breast cancer), A549 (lung cancer), etc. |
| Primary Cells | Primary hepatocytes, primary neurons (neurodegenerative disease modeling), primary fibroblasts (fibrosis modeling), etc. |
| Stem Cells and Organoids | iPSCs-derived cardiomyocytes/neurons (regenerative medicine), intestinal/liver organoids, etc. |
| Immune Cells | T cells/B cells, macrophages, etc. |
BOC Sciences leverages advanced instrumentation platforms to ensure the high efficiency and accuracy of cell proliferation and activity testing services. Equipped with high-performance detection and imaging systems, we can capture dynamic changes in cell proliferation, viability, and cell cycle progression with exceptional sensitivity. Our integration of high-throughput analysis and automated cell handling platforms significantly enhances experimental accuracy and reproducibility. Meanwhile, optimized cell culture and processing environments further ensure the stability of cells and the consistency of results, accelerating drug development and advancing life science research.
| Flow Cytometer | Live Cell Imaging System |
| Fluorescence Microscope | Cell Counter |
| ELISA Reader | CO2 Incubator |
| Confocal Microscope | Centrifuge |
BOC Sciences offers a broad range of cell proliferation and activity testing services designed to meet diverse research needs. Our advanced technologies help researchers gain accurate insights into cellular behavior and the effects of various treatments, from drug discovery to stem cell research. With our versatile testing methods, we provide reliable, reproducible results for your cell-based assays.
Utilizing instruments such as the hemocytometer or automated cell counters (e.g., CASY-TTC), this method directly counts cells, offering a clear indication of cell proliferation. For example, monitoring cell count at various time points (24, 48, and 72 hours) helps analyze the effect of a drug on cell growth by comparing treated versus control groups.
The MTT assay is a widely used method that assesses cell viability by measuring the reduction of MTT to purple formazan crystals by mitochondrial enzymes. Cell number and proliferation show a direct relationship with the color intensity measured at 570 nm. Researchers find this method particularly effective for examining cellular reactions to growth factors, pharmaceuticals and various stimuli.
Like MTT, the CCK-8 assay utilizes WST-8 that mitochondrial dehydrogenases in living cells transform into a water-soluble yellow formazan dye. Cell proliferation measurement occurs through the intensity analysis of yellow color produced at 450 nm. CCK-8 stands out as a better option for high-throughput screenings because it offers greater sensitivity and easier usage than MTT and eliminates the need for a solubilization step.
During the S phase when cells synthesize DNA they integrate BrdU which functions as a thymidine analog. The identification of BrdU uptake via immunocytochemistry or antibody-based techniques establishes which cells are undergoing division, providing critical insights into cancer cell growth dynamics and cell cycle examination.
The DNA incorporation of EdU during cell division follows the same pattern as BrdU does. EdU detection differs from BrdU detection because it uses a click-chemistry-based fluorescent labeling technique which provides enhanced sensitivity and a simpler procedure. Rapid and accurate evaluation of DNA synthesis in dividing cells can be achieved through this method.
The DNA content analysis performed by flow cytometry employs DNA-binding dyes including propidium iodide (PI). The method delivers precise information about different cell cycle phases (G1, S, G2/M) and assesses treatment effects on cell cycle progression by revealing potential drug-induced G1 phase cell cycle arrest which blocks cell division.
ATP is a key indicator of cellular metabolism and vitality. ATP content can be measured using a luciferase-based assay system, providing a reliable reflection of cellular activity. This method is frequently used in cell metabolic studies to quickly assess the impact of nutritional changes or drug treatments on cellular energy metabolism.
LDH is an enzyme found in the cytoplasm that leaks into the extracellular medium when cells are damaged or die. By measuring LDH activity in the culture medium, this assay provides insights into cytotoxicity and cell damage. It’s often used in toxicity testing to evaluate the effects of pharmaceuticals, chemicals, or toxins on cell integrity.
Understanding Requirements
Communicate with the client to clarify their research goals, including selecting specific cell lines, evaluating cell proliferation activity, or studying the effects of different compounds on cells, ensuring the test aligns with their research objectives.
Experimental Design
Develop a detailed experimental plan based on the client's needs, selecting suitable detection methods (such as colorimetric or fluorescence assays) to assess cell viability, proliferation, and metabolic activity, while establishing experimental conditions.
Experimental System Construction
Prepare the required cell cultures, reagents, and assay systems, ensuring optimal system conditions to maintain stability throughout the experiment and achieve accurate measurement results.
Experimental Testing
Perform cell proliferation tests, such as MTT, WST-1, or cell counting assays, to assess the impact of compounds on cell growth and measure key parameters.
Data Collection and Analysis
Collect experimental data and organize it while analyzing cell proliferation rates, compound effects, dose-response relationships and cell activity markers to maintain comprehensive and comparable datasets.
Report Delivery
Analyze the collected data and generate a detailed test report, including proliferation curves, cell activity analysis, and observed cell morphological changes, providing valuable research insights and recommendations for improvement.
BOC Sciences' cell proliferation and activity assay services cover a wide range of applications designed to assess cell growth, viability, and functional activity. Our services include evaluating the effects of various compounds on cell proliferation, metabolic activity, and overall cellular health. We utilize advanced assay techniques to measure cell division, enzyme activity, ATP production, and other key parameters, providing valuable insights for drug discovery, toxicology studies, and biomedical research. Whether for screening therapeutic agents, understanding cellular responses, or optimizing drug candidates, our assays deliver accurate and reproducible results to support your research and development needs.
| Target Category | Targets | Application Diseases |
| Cancer Targeted Therapy | EGFR, HER2, ALK, BRAF, VEGFR, MET, ROS1, RET, PI3K, mTOR, etc. | Non-small cell lung cancer, breast cancer, melanoma, renal cancer, colorectal cancer, etc. |
| Immunomodulatory Therapy | JAK1/2/3, TYK2, BTK, SYK, IL-6R, PD-1, PD-L1, CTLA-4, etc. | Rheumatoid arthritis, psoriasis, systemic lupus erythematosus, inflammatory bowel disease, etc. |
| Anti-Infective Therapy | HCV NS5B, HIV reverse transcriptase, HIV integrase cofactors, TLR pathways, bacterial resistance-related kinases, etc. | Hepatitis C, HIV/AIDS, multidrug-resistant bacterial infections, etc. |
| Neurological Disease Therapy | CDK5, GSK-3β, LRRK2, Tau-related kinases, dopamine receptors D1/D2, etc. | Alzheimer's disease, Parkinson's disease, schizophrenia, depression, etc. |
| Metabolic Disease Therapy | GLP-1R, PPARα/γ, AMPK, mTOR, PI3K-Akt, SREBP, JNK, etc. | Type 2 diabetes, fatty liver disease, obesity, metabolic syndrome, etc. |
| Anti-Inflammatory and Anti-Fibrosis Therapy | p38 MAPK, ROCK, TAK1, TGF-β pathway, NF-κB pathway, etc. | Idiopathic pulmonary fibrosis, liver fibrosis, rheumatoid arthritis, etc. |
| Cardiovascular Disease Therapy | VEGFR, PDGFR, eNOS, AMPK, natriuretic peptide receptors, etc. | Atherosclerosis, heart failure, hypertension, coronary heart disease, etc. |
| Hematological Disease Therapy | BCR-ABL, FLT3, JAK2, KIT, CSF1R, etc. | Chronic myeloid leukemia, acute myeloid leukemia, myelofibrosis, etc. |
| Dermatological and Wound Healing Therapy | IL-17, TNF-α, EGFR, TGF-β, etc. | Psoriasis, atopic dermatitis, wound healing, scar treatment, etc. |
| Anti-Aging and Rare Disease Therapy | Senescence-related pathways (p16, p53, mTOR), CFTR, SMN1, etc. | Progeria syndrome, cystic fibrosis, spinal muscular atrophy, etc. |
What is a cell proliferation assay?
A cell proliferation assay measures the ability of cells to divide and multiply. This test helps assess cell growth, survival, and response to various stimuli or treatments, often used in drug screening, cancer research, and toxicology studies. It is crucial for evaluating the effects of new compounds on cell growth and identifying potential therapeutic agents.
How does a cell activity assay work?
A cell activity assay measures the metabolic or functional activity of cells, such as enzyme activity, ATP production, or cellular respiration. These assays provide insights into the overall health, viability, and response of cells to specific treatments, making them essential for evaluating the efficacy and safety of drugs, vaccines, or biologics.
What are the benefits of cell proliferation testing in research?
Cell proliferation testing is vital in understanding how cells grow and respond to various conditions. It is commonly used to evaluate the effects of drugs, toxins, and growth factors, helping researchers determine whether a compound promotes or inhibits cell division. This is crucial in cancer research, drug discovery, and stem cell studies.
Why is measuring cell activity important in drug development?
Measuring cell activity is crucial in drug development because it allows researchers to assess how potential drug candidates influence cellular functions. By monitoring parameters like enzyme activity, ion channel behavior, or metabolic changes, researchers can identify compounds that improve cellular health or target specific diseases, aiding in the development of more effective therapies.
