The analysis and identification of chiral compounds are one of the hot issues in the current analytical chemistry community. The chiral enantiomers are very similar in all aspects of their physical and chemical properties, so their separation is a difficulty in separation science. At the same time, chirality plays an extremely crucial role in the life sciences, because many of the major biological activities are achieved through strict chiral matching to generate molecular recognition. The main analytical method for chiral separation is chromatographic separation analysis.
High-performance liquid chromatography(HPLC) has a wide range of applications and strong separation capability, and it has become one of the preferred technology platforms for the separation of chiral compounds. Supercritical fluid chromatography (SFC) is a new chromatographic technique. Because it has the advantages that gas and liquid phases do not have, it can separate and analyze some objects that gas and liquid chromatography cannot solve.
Fig.1 Analysis of a compound in HPLC
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The method for identifying compounds by HPLC and SFC is basically the same, and it is to determine what each chromatogram peak represents, and thus determine the composition of the sample mixture made up of these components. With pure substances as a control, each substance has its own retention value (including retention time, retention volume, adjustment of retention time, adjustment of retention volume, etc.) under certain chromatographic conditions. First determine the retention time or retention distance of each chromatographic peak in the unknown, then inject a pure substance to be measured into the chromatograph, compare and compare the retention values of the two, if the two retention values are the same, then the unknown can be determined. Pure substances are known to be the same substance. It is also possible to add a pure substance directly into the sample and then into the chromatograph. If the peak height of a component peak increases, it means that this chromatographic peak is the pure substance added. This method is also called the pure substance addition method. In general, HPLC and SFC supported columns can separate isomeric mixtures with multiple chiral atoms, but if the two enantiomers differ very little, ordinary operations are difficult to distinguish, then you need to use excellent results. Chiral column specifically designed to deal with this situation.
BOC Sciences has advanced equipment and professional technicians who can provide you with analytical and appraisal services for specific chiral compounds. If you need services in this area, please contact us. We will wholeheartedly serve you.