BOC Sciences is a professional service provider of chiral compound analysis and identification at home and abroad. We not only have professional technical level, but also good after-sales service and high-quality solutions.
Chirality plays an extremely crucial role in life sciences because many major biological activities are achieved through strict chiral matching to generate molecular recognition. Therefore, it is undoubtedly of great significance to study the chiral molecular system and mechanism of action and continuously develop new technologies and new methods for chiral research.The analysis and identification of chiral compounds are one of the hot issues in the current analytical chemistry community. Modern chromatographic separation technology shows great superiority in the separation and determination of enantiomers.
HPLC is the most widely used method for the separation and determination of enantiomers. It is especially suitable for the analysis of chiral materials with strong polarity and poor thermal stability. Supercritical fluid chromatography (SFC) is a new chromatographic technique. Because it has the advantages that gas and liquid phases do not have, it can separate and analyze some objects that gas and liquid chromatography cannot solve.
Fig.1 Analysis of a compound in HPLC
HPLC uses a high-pressure infusion system to pump single or mixed solvents, buffers and other liquid mobile phases with different polarities into a chromatographic column equipped with a stationary phase. The components are separated in the chromatographic column, and then enter the detector for detection, so as to realize the analysis and identification of the sample. The identification and quantification of compounds in HPLC are determined by chromatograms. Usually, the x-axis represents retention time, and the y-axis depends on the detection method. An ultraviolet detector is used to measure the intensity of absorbance. In addition, other types of detectors, such as mass spectrometry (MS), nuclear magnetic resonance (NMR), and fluorescence detectors, can also be used.
The method for identifying compounds by HPLC and SFC is basically the same. It is to determine what each chromatogram peak represents, and thus determine the composition of the sample mixture made up of these components. The retention value is related to the structure and properties of the component and can be used for compound identification. With pure substances as a control, each substance has its own retention value (including retention time, retention volume, adjustment of retention time, adjustment of retention volume, etc.) under certain chromatographic conditions. First determine the retention time or retention distance of each chromatographic peak in the unknown, then inject a pure substance to be measured into the chromatograph, compare the retention values of the two. If the two retention values are the same, then the unknown can be determined. Pure substances are known to be the same substance. It is also possible to add a pure substance directly into the sample and then into the chromatograph. If the peak height of a component peak increases, it means that this chromatographic peak is the pure substance added. This method is also called the pure substance addition method. In general, HPLC and SFC supported columns can separate isomeric mixtures with multiple chiral atoms. But if the two enantiomers differ very little, ordinary operations are difficult to distinguish, then you need to use excellent results. The chiral column is specifically designed to deal with this situation.
BOC Sciences has advanced equipment and professional technicians who can provide you with analytical and appraisal services for specific chiral compounds. If you need services in this area, please contact us. We will wholeheartedly serve you.
How do you identify enantiomers in chemical analysis?
Enantiomers can be identified using chiral separation techniques such as high-performance liquid chromatography (HPLC) with chiral stationary phases or supercritical fluid chromatography (SFC). These methods separate enantiomers based on their differential interactions with the chiral medium, allowing for individual characterization.
How can you determine if an enantiomer is R or S configuration?
The R/S configuration is determined by applying the Cahn–Ingold–Prelog priority rules to the chiral center and analyzing the spatial arrangement of substituents. Experimental determination can be supported by chiroptical methods such as optical rotation, circular dichroism, and chiral chromatography with reference standards.
How do you identify the enantiomer of each compound in a mixture?
To identify each enantiomer in a mixture, chiral chromatography is often employed to achieve baseline separation, followed by spectral or spectrometric analysis. Comparison with known standards or derivatization with chiral reagents can confirm enantiomeric identity.
How to distinguish D and L enantiomers in research?
D and L notation refers to the stereochemical configuration relative to a reference molecule such as glyceraldehyde. Determination often involves chiral chromatographic separation, optical rotation measurement, and correlation with established stereochemical data.
What enantiomer identification capabilities does BOC Sciences provide?
BOC Sciences offers comprehensive enantiomer identification and analysis services using advanced HPLC and SFC platforms. Our capabilities include chiral separation, optical purity determination, and structural assignment for a wide range of chiral compounds.
Our enantiomer identification services use advanced chromatographic techniques for accurate determination of stereochemistry. BOC Sciences ensures clear, reproducible results.
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