Peptide Synthesis

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Peptide Synthesis

Peptides are central compounds in the pharmaceutical industry, providing both final medications and lead compounds for the preparation of peptidomimetic or non-peptidic pharmaceuticals. Therapeutic peptides are recognised as being extremely specific in their binding to in vivo targets, resulting in them often being highly potent and very selective, whilst having very few negative side effects. Currently, there are more than 60 peptide based medicines approved for use by the US Food & Drug Administration, around 140 peptide drugs currently in clinical trials and over 500 in preclinical trials.

Below is a list of our Peptide Synthesis Services (include but not limited to the following):

Peptide design

Before any meaningful studies about proteins and peptides can be performed, it is important to consider several factors. The length of sequence, amino acid residues and the sequence of peptides will influence whether correct assembly and purification are feasible. So we need some strategies to design peptide better.

  • Reduce the length of sequence
  • Reduce the number of hydrophobic residues
  • Reduce difficult residues
  • Change the sequence by choosing a different “framework”

Many synthetic peptides are important commercial or pharmaceutical products ranging from aspartame a dipeptide-sugar substitute to clinically used hormones such as oxytocin adrenocorticotropic hormone and calcitonin. The chemical synthesis of peptides is the condensation of the carboxyl group of one amino acid with the amino group of another amino acid. Protective group strategies are usually necessary to prevent adverse side effects with various amino acid side chains. Thousands of special modifications including: Phosphorylation, Fluorescent dyes, Glycosylation, PEGylation, Rhodamine, Biotin, D-amino acids, stable isotopes, EDANS, Dabsyl, Dansyl, Abz, thiolactic acids, etc.

Although peptide synthesis strategies have been optimized and can be mass produced, the process of peptide synthesis is not perfect. Events such as incomplete deprotection or reactions with free protective groups may result in truncation or deletion of sequences isomers or other by-products. Purification strategies are often based on a combination of separation methods that take advantage of the physicochemical properties of peptides, including size, charge, and hydrophobicity. Purification technologies include:

  • Size exclusion chromatography.
  • Ion exchange chromatography (IEC).
  • Partition chromatography.
  • High performance liquid chromatography (HPLC).

Why choose BOC Sciences?

BOC Sciences is specialized in the peptide synthesis services, providing a confidential and efficient service at competitive prices. Every step of peptide synthesis is subject to BOC Sciences’ stringent quality control. Typical delivery specifications include:

  • Synthesis of peptides either as crude products or as HPLC-purified material
  • Personalized Ph.D. level consultation with experienced peptide chemistry experts
  • Peptides from 2 to 135 Amino Acids
  • Scales from Nanomole to Milligram to Gram to Kilogram
  • In quantities of several milligrams up to 100 grams
  • Quality assurance: HPLC chromatogram and Mass spec analysis
  • Cyclic Peptides - Cys to Cys, Head to Tail, Internal Lactam
  • Stapled peptides
  • High Throughput Synthesis
  • Additional analyses, such as stability determination, amino acid analysis, and residual solvents etc.
  • Rush Service is Available

References

  1. Benoiton, N. L. (2016). Chemistry of peptide synthesis. CRC Press.
  2. Behrendt, R., White, P., & Offer, J. (2016). Advances in Fmoc solid‐phase peptide synthesis. Journal of Peptide Science22(1), 4-27.
  3. Benoiton, N. L. (2016). Chemistry of peptide synthesis. CRC Press.
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