Purification technology is a fundamental component of drug development, enabling the isolation and refinement of target compounds to achieve high purity and functionality. It plays a vital role in ensuring the quality, consistency, and performance of pharmaceutical substances throughout the discovery and production process. BOC Sciences integrates state-of-the-art purification techniques with extensive technical expertise to deliver comprehensive purification services. Our advanced instrumentation and optimized protocols guarantee efficient separation and recovery of diverse molecules. By continuously advancing purification methodologies and maintaining rigorous process control, BOC Sciences serves as a reliable partner in purification solutions, supporting researchers in accelerating the development of high-quality therapeutic candidates.
Purification technology plays a crucial role across various industries, including pharmaceuticals, cosmetics, agriculture, and biotechnology. However, researchers and manufacturers frequently face persistent challenges that impact efficiency and outcomes. Choosing the most suitable purification method for complex and diverse samples can be time-consuming and technically complex, often resulting in insufficient purity or low recovery rates. Many purification techniques require sophisticated equipment and specialized expertise, which may be unavailable in some laboratories or production facilities. Scaling up purification processes while maintaining consistency and cost-effectiveness remains a significant hurdle. Additionally, thorough characterization of purified products, including impurity profiling, aggregate analysis, and structural verification, demands advanced analytical capabilities and interdisciplinary knowledge. Limited personnel and tight deadlines further strain operational workflows. Furthermore, complex internal procedures, such as multi-stage approvals, extensive documentation, and intricate process management, often cause delays and reduce overall efficiency.
We offer a full suite of purification techniques, including chromatography, filtration, crystallization, extraction, and electrophoresis, covering diverse molecular types such as small molecules, peptides, proteins, antibodies, nucleic acids, lipids, and polymers.
Our purification workflows are adaptable from milligram-scale R&D to multi-gram pilot and production batches, ensuring smooth scale-up and consistent product quality.
Coupled with advanced analytical platforms, we provide robust in-process control, impurity profiling , purity testing , and structural validation to ensure high-purity output and process traceability.
With streamlined operations and expert teams, we respond quickly to client needs, enabling efficient project initiation, execution, and delivery without compromising precision.
BOC Sciences offers a full suite of advanced purification technology solutions that support biopharmaceutical research and development from early discovery through process scale-up. Our platform integrates both classical and cutting-edge separation techniques to ensure high purity, efficiency, and scalability across a broad range of molecule types, including small molecules, peptides, and biologics.
We offer flash column chromatography for rapid and scalable purification of organic compounds. Utilizing a range of stationary phases and gradient systems, this technique enables efficient separation of complex mixtures, supporting intermediate purification and synthetic process optimization.
Our preparative high-performance liquid chromatography (HPLC) services deliver high-resolution purification of small molecules, peptides, and polar compounds. With diverse column chemistries and optimized mobile phase systems, we ensure high recovery, purity, and method scalability from milligram to gram levels.
We apply recrystallization techniques to refine compound purity and control solid form properties. Through solvent system screening and temperature gradient optimization, our services support API purification, polymorph selection, and formulation stability in early and late-stage development.
We employ affinity chromatography techniques for the highly selective separation of target biomolecules. Utilizing ligands such as Protein A/G, metal ions (IMAC), or specific antigens, our services are ideal for purifying antibodies, fusion proteins, and enzymes with exceptional binding specificity and recovery yields.
Our ion exchange chromatography (IEC) services leverage charge-based interactions for the purification of proteins, nucleic acids, and other charged biomolecules. With both cationic and anionic exchange capabilities, we optimize separation conditions for maximum resolution and throughput in both analytical and preparative scales.
RP-HPLC is employed for small molecule, peptide, and impurity profiling. We offer scalable RP-HPLC purification with high resolution, excellent reproducibility, and robust process consistency to support downstream applications.
Our SEC services provide precise molecular weight-based separation for desalting, buffer exchange, and aggregate removal in protein formulations. This technique is critical for downstream analysis and formulation development.
We offer LLE solutions for isolating active pharmaceutical ingredients (APIs) from complex matrices. Our method development ensures optimal partitioning behavior and solvent system compatibility to achieve high recovery rates and scalability.
We utilize electrophoretic techniques, including SDS-PAGE and capillary electrophoresis (CE), to analyze protein purity and size distribution. These methods support analytical characterization and validation during preclinical development.
Our SPE services are used for sample cleanup and concentration in bioanalysis workflows. With various cartridge chemistries, we provide tailored solutions for matrix removal and analyte enrichment in both biological and synthetic samples.
We apply ultrafiltration and dialysis techniques for buffer exchange, desalting, and molecular weight cut-off-based separations. These techniques are particularly useful for protein purification and formulation studies.
We integrate membrane filtration systems, including microfiltration, nanofiltration, and tangential flow filtration (TFF), into our purification platforms to support continuous processing and high-throughput purification in bioprocessing environments.
BOC Sciences provides customized purification method development tailored to your sample characteristics and project needs. Our team works closely with clients to design optimal strategies using a broad range of chromatographic, membrane, and extraction technologies. We evaluate key attributes, such as molecular weight, solubility, and binding affinity, to recommend and validate the most effective purification approach for small molecules, peptides, nucleic acids, or biologics. From R&D to scale-up, we ensure high purity, recovery, and process scalability. BOC Sciences is dedicated to delivering tailored solutions that enhance efficiency and support smooth drug development progression.
BOC Sciences provides a robust suite of analytical and validation services tailored to complement our purification solutions. Our advanced platforms support precise purity evaluation, impurity profiling, residual detection, and conformational integrity assessments across various drug modalities. These services are essential for ensuring product quality, structural fidelity, and downstream application readiness in drug research and development.
| Service Category | Description |
| Structure Characterization | Molecular structure determination, conformational analysis |
| Purity Determination | Quantitative purity testing via HPLC, CE, UV-Vis |
| Element Analysis | Quantification of elemental composition, counter ions |
| Impurity Isolation and Identification | Detection and profiling of impurities using LC-MS/MS |
| Heavy Metal Analysis | Trace metal quantification by ICP-MS |
| Counter Ion Analysis | Identification and quantification of counter ions |
| Thermal Analysis | Thermal stability and transition studies (DSC, TGA) |
| Stability Studies | Evaluation of chemical and physical stability under various conditions |
BOC Sciences' purification service platform is built on a comprehensive suite of advanced technologies and instrumentation, designed to support high-efficiency separation and high-purity recovery across a broad spectrum of drug substances. Our integrated chromatographic, filtration, extraction, and analytical systems enable flexible and effective purification solutions for diverse molecular types. We are committed to delivering precise and reliable purification services that facilitate the smooth progress of drug discovery and development projects.
At BOC Sciences, we offer a complete range of purification solutions tailored to support drug screening workflows across various molecular modalities. Whether you are working with small molecules, peptides, proteins, nucleic acids, or complex biomaterials, our platform integrates advanced separation technologies to ensure high-purity output and process efficiency. Below are some of the key purification techniques we provide:
We provide scalable purification services for small-molecule drugs using flash chromatography, preparative HPLC, and crystallization. Our solutions ensure optimal recovery, purity, and structural integrity suitable for bioassays and formulation.
Our peptide purification services leverage RP-HPLC and ion exchange chromatography to deliver high-purity peptides. We offer method development, impurity profiling, and scale-up services for therapeutic peptides and peptide libraries.
Using a combination of affinity chromatography, SEC, and ultrafiltration, we provide robust purification workflows for proteins across expression systems. These services support structural studies, functional assays, and preclinical testing.
We utilize Protein A/G affinity chromatography, HIC, and polishing steps for monoclonal and polyclonal antibody purification. Our platform ensures high yield, low impurity levels, and scalability from milligram to gram quantities.
Our nucleic acid purification services encompass plasmid DNA, mRNA, and siRNA workflows. Techniques include anion exchange chromatography, ethanol precipitation, and TFF, ensuring purity and integrity for transfection and therapeutic use.
We offer DNA isolation and purification using silica membrane, magnetic beads, and spin-column methods. These services deliver high-quality DNA suitable for PCR, cloning, sequencing, and gene expression studies.
Our lipid purification services use techniques such as normal-phase chromatography and solvent extraction to isolate and purify natural and synthetic lipids. Applications include formulation development, lipidomic, and nanoparticle delivery systems.
We support the purification of synthetic and natural polymers through precipitation, dialysis, SEC, and membrane-based techniques. These services enable the preparation of polymers for drug delivery, biomaterials, and analytical studies.
Collaborate with clients to grasp drug properties, purity targets, and project needs. Identify molecule types, purity criteria, scalability, and downstream uses to design appropriate purification strategies.
Create a tailored purification plan selecting suitable methods, chromatography, filtration, crystallization, based on compound traits and purity goals. Define key parameters and timelines.
Optimize small-scale purification by screening techniques to maximize yield and purity. Assess recovery, impurities, and aggregation, refining the approach as needed.
Conduct scaled purification with validated protocols, monitoring critical parameters to ensure consistency and reproducibility.
Perform purity, impurity, residual contaminant, and structural integrity analyses using HPLC, LC-MS, ELISA, SEC-HPLC, and thermal methods to confirm process effectiveness.
Provide purified compounds with full analytical reports and process documentation to facilitate subsequent drug development stages.
A purification technique involves methods to separate and isolate a specific target compound or biomolecule from a complex mixture, improving the purity and quality of the substance for research and production purposes.
Common methods include chromatography (affinity, ion exchange, reversed-phase HPLC), filtration (ultrafiltration, microfiltration), crystallization, electrophoresis, and liquid-liquid extraction. The choice depends on the properties of the target and impurities.
Chromatography separates mixture components based on their interaction differences with stationary and mobile phases. Different modes, like affinity or ion exchange, target specific molecule properties, enabling effective purification.
Purification removes impurities and contaminants to obtain a high-purity product, ensuring accurate evaluation of biological activity and reliable characterization of compounds during research and production.
