Antibody Conjugation

Antibody Conjugation

BOC Sciences has unique R&D expertise to provide the highest quality custom antibody conjugation services of different sizes and reasonable prices in the industry. At the same time, we provide a comprehensive analysis of antibody conjugates. BOC Sciences's methods can handle powerful and toxic payloads. In the process of development and production, each antibody conjugate is strictly monitored according to our strict quality assurance and quality control standards.

Introduction

Antibody conjugation play an important role in biological agents targeted therapeutic studies, and immunoassay or detection applications. The custom antibody bioconjugation services can be achieved by using monoclonal antibody or polyclonal antibody through chemical conjugation with other biomolecules. One method is the conjugation of antibodies and their fragments with biologically active molecules, including nucleic acids, oligonucleotides and their analogs, peptides, proteins. Another way is the conjugation of antibodies and their fragments with any molecular group that adds valuable properties such as radionuclides, drugs, toxins, enzymes, metal chelates, fluorophores, haptens, and others.

Below is a list of our Antibody Conjugates Services (include but not limited to the following):

Antibody-enzyme conjugation is designed as a new approach for disease therapy, especially for cancer therapy. Conjugation of enzymes to antibodies is the formation of a stable, covalent linkage between an antigen‐specific monoclonal or polyclonal antibody and enzyme, such as horseradish peroxidase (HRPO), urease, or alkaline phosphatas.

More than a century ago, Paul Ehrlich proposed the concept of “antibody-drug conjugates (ADCs)” as one of the targeted therapies. Now ADCs are being regarded as very important and promising antitumor agents. There are four conjugates that have been approved by regulatory agencies for treating cancer patients. The typical method is the conjugation of cytotoxins or chemotherapeutic drugs (such as cysteine, glutamine, unnatural amino acids, short peptide tags, and glycans) with specifically defined sites in antibody molecules.

A large number of polymers have the ability to conjugate with antibodies. Antibody conjugation to polymers directed against tumor-associated antigens is a new research area for scientists. The drug delivery systems, composed of polymers and their conjugates with antibodies, fragments of antibodies, or oligopeptides, are suitable as carriers mainly for anticancer or anti-inflammatory agents.

The size of the antibody allows multiple fluorophores to attach to a single antibody, which breaks the strength limit of a single fluorophore. The coupling between the fluorophore and the antibody can be realized by the specific interaction between the functional groups on the two components. The antibody fluorescent probe not only facilitates the sorting and quantification of surface antigens, but also provides a means to obtain high-resolution images of cells carrying antibody binding targets.

Among a wide range of targeted ligands, antibodies and their derivatives have been proved to be effective as imaging agents. Nanoparticles of nanoprobes or drug carriers are directed to their desired sites in the body. Compared with free nanoparticles, antibody-coupled nanoparticles have higher site specificity, resulting in higher accumulation in the target region, so the dose requirement is lower. Nano-antibodies can be used in the treatment of tumors and other diseases, disease detection, vaccine development and so on.

Chelated magnetic ion complexes enable them to further combine with functional biomaterials, including antibodies, to produce antibody-magnetic ion probes, which can enhance the imaging contrast of certain tissues/organs in the body that present the target antigen. and lead to a more effective diagnosis of specific diseases.

AAC has the key characteristics of antibodies and antibiotics. Compared with antibodies, it shows enhanced ADME (adsorption, distribution, metabolism, excretion) and excellent target specificity. Studies have shown that AAC is expected to show the efficacy and specificity of improving multi-drug resistant bacteria, and will open up a new way for the discovery of antibiotics in the future.

Biotin labeled antibody technique is a technique for labeling biotin on specific antigens or antibody molecules. This enhanced amplification effect can show the characteristics and content of antigens or antibodies in the immune response system. It has been widely used in the analysis and technical determination of medical pathology, immunohistochemistry, molecular biology, biopharmaceutical and other fields.

We also provide complete characterization services for antibody conjugates:

  • Advanced Analysis method: HPLC (HIC,SEC,RP), iCIEF,LC-MS.
  • The analysis covers all stages of development and research: drug-to-antibody ratio(DAR), ADC affinity, protein concentration, peptide map analysis, and efficacy testing, etc.
  • In vitro pharmacology: more than 100 common cancer cell lines (including the NCI-60 group) are used in cytotoxicity studies.
  • The scale of production ranges from 1 mg to 10 g, which can be used for in vitro and in vivo research.

References

  1. Grüttner, C., Müller, K., Teller, J., Westphal, F., Foreman, A., & Ivkov, R. (2007). Synthesis and antibody conjugation of magnetic nanoparticles with improved specific power absorption rates for alternating magnetic field cancer therapy. Journal of Magnetism and Magnetic Materials, 311(1), 181-186.
  2. Orelma, H., Teerinen, T., Johansson, L. S., Holappa, S., & Laine, J. (2012). CMC-modified cellulose biointerface for antibody conjugation. Biomacromolecules, 13(4), 1051-1058.
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