Antibody Conjugation

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Antibody Conjugation

Antibody conjugates play an important role in biological agents targeted therapeutic studies, and immunoassay or detection applications. The custom antibody bioconjugation services can be achieved by using monoclonal antibody or polyclonal antibody through chemical conjugation with other biomolecules. One method is the conjugation of antibodies and their fragments with biologically active molecules, including nucleic acids, oligonucleotides and their analogs, peptides, proteins. Another way is the conjugation of antibodies and their fragments with any molecular group that adds valuable properties such as radionuclides, drugs, toxins, enzymes, metal chelates, fluorophores, haptens, and others.

Below is a list of our Antibody Conjugates Services (include but not limited to the following):

Antibody-enzyme conjugation is designed as a new approach for disease therapy, especially for cancer therapy. Conjugation of enzymes to antibodies is the formation of a stable, covalent linkage between an antigen‐specific monoclonal or polyclonal antibody and enzyme, such as horseradish peroxidase (HRPO), urease, or alkaline phosphates.

More than a century ago, Paul Ehrlich proposed the concept of “antibody-drug conjugates (ADCs)” as one of the targeted therapies. Now ADCs are being regarded as very important and promising antitumor agents. There are four conjugates that have been approved by regulatory agencies for treating cancer patients. The typical method is the conjugation of cytotoxins or chemotherapeutic drugs (such as cysteine, glutamine, unnatural amino acids, short peptide tags, and glycans) with specifically defined sites in antibody molecules.

A large number of polymers have the ability to conjugate with antibodies. Antibody conjugation to polymers directed against tumor associated antigens is a new research area for scientists. The drug delivery systems, composed of polymers and their conjugates with antibodies, fragments of antibodies or oligopeptides, is suitable as carriers mainly for anticancer or anti-inflammatory agents.

Conjugated is the process of covalently connecting two molecules/polymers or biomolecules/ biopolymers. Studies have shown that antibody molecules have many functional groups suitable for modification or binding. Cross-linked oligonucleotides antibodies can be realized by lysine and N-terminal groups.

More than 60 years ago, Coons and his partners first reported the coupling of fluorescent parts with antibodies to produce labeled antibody reagents, which has become a routine and important step in bioscience and medicine. Labeling antibodies with fluorescent dyes provides a colored reagent that can be directly observed. The intensity and narrow wavelength of fluorescent dye make fluorescent dye a useful tool in immunochemistry. Many of our tags can be used with multiple antibodies that do not cross-react to create customized multiple detection methods.

Why Choose BOC Sciences?

BOC Sciences provides high-quality, low-cost, high-tech products to customers around the world. We has employed a dedicated staff of professional chemists to help you develop the most efficient process for synthesis of antibody conjugations. With diverse state-of-the-art technologies and approaches, clients’ synthetic route can be developed and optimized in the most cost effective way. Each step of product synthesis is subject to BOC Sciences’ stringent quality control.

References

  1. Grüttner, C., Müller, K., Teller, J., Westphal, F., Foreman, A., & Ivkov, R. (2007). Synthesis and antibody conjugation of magnetic nanoparticles with improved specific power absorption rates for alternating magnetic field cancer therapy. Journal of Magnetism and Magnetic Materials311(1), 181-186.
  2. Orelma, H., Teerinen, T., Johansson, L. S., Holappa, S., & Laine, J. (2012). CMC-modified cellulose biointerface for antibody conjugation. Biomacromolecules13(4), 1051-1058.
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